代谢综合征基础与临床系列研究——GRGDSP/7E3对动脉粥样硬化内皮细胞MMPs的mRNA表达的影响

目的了解GRGDSP/7E3对基质金属蛋白酶（MMP-2,MMP-9）mRNA表达的影响。方法体外培养人脐静脉内皮细胞,分别用酶谱法和逆转录聚合酶链式反应（RT-PCR）检测MMP-2,MMP-9活性和mRNA表达。待细胞融合成单层后,分别加入无血清培养基、静息期血小板、活化后血小板及interleukin-1β（IL-1β）,共同培养60min后,洗去血小板,内皮细胞继续培养6h。上清液及细胞分别用于酶谱法检测MMP-2和MMP-9活性,以及RT-PCR分析mRNA表达。结果内皮细胞与活化血小板共同培养后,酶谱法显示：与对照组相比,未活化的血小板轻微诱导MMP-2和MMP-9分泌,而活化状态的血小板能显著诱导这二者的分泌,与IL-1β的作用相当;而且还可以见到62kDaMMP-2活化形式。加入阻断剂GRGDSP以及GPⅡb/Ⅲa单克隆抗体（7E3）作用后检测发现,MMP-2和MMP-9分泌减少。与活化血小板共同培养后的内皮细胞表面表达uPAR和MT1-MMP以及上清液中MMP-2的mRNA与对照组相比明显增高,而静息状态的血小板作用不显著。加入GRGDSP或7E3后,mR-NA的表达降低。结论①活化状态的血小板与IL-1β的作用相当,能显著诱导MMP-2和MMP-9分泌;能明显增高内皮细胞（EC）-uPAR和MT1-MMP及上清液中MMP-2mRNA的表达;②GPⅡb/Ⅲa阻断剂可能抑制血小板在不稳定斑块部位聚集、粘附等一系列炎症反应。     

基于SVM的综合实力评估系统研究

简略介绍了支持向量机的基本思想 ,研究其在综合评价中的应用。分析了对大学进行实力评估的过程 ,建立基于SVM的评价系统。比较新评价系统和采用其他方法如PCA (PrincipalComponentAnalysis主元分析 ) ,Fisher等建立的评价系统所分别取得的拟合效果。结果表明 :采用支持向量机设计的评价系统思路清晰 ,操作简单并且能取得更为理想的评估结果。     

中国私营经济发展的障碍因素分析

改革开放以来的20年,是我国私营经济发展的最好时期。私营经济已成为中国经济新的增长源,必将为中国的经济做出突出贡献。但从目前的现状看,影响私营经济发展的障碍因素,依旧严重地制约着私营经济的进一步发展。因此,客观地评价私营经济发展的历史、冷静地分析它的现状、科学地提出对策建议,将有利于私营经济的新发展,使其进入到一个更高的发展阶段。     

Applicability of a Neuroprobabilistic Integral Risk Index for the Environmental Management of Polluted Areas: A Case Study

Recently, we developed a GIS-Integrated Integral Risk Index (IRI) to assess human health risks in areas with presence of environmental pollutants. Contaminants were previously ranked by applying a self-organizing map (SOM) to their characteristics of persistence, bioaccumulation, and toxicity in order to obtain the Hazard Index (HI). In the present study, the original IRI was substantially improved by allowing the entrance of probabilistic data. A neuroprobabilistic HI was developed by combining SOM and Monte Carlo analysis. In general terms, the deterministic and probabilistic HIs followed a similar pattern: polychlorinated biphenyls (PCBs) and light polycyclic aromatic hydrocarbons (PAHs) were the pollutants showing the highest and lowest values of HI, respectively. However, the bioaccumulation value of heavy metals notably increased after considering a probability density function to explain the bioaccumulation factor. To check its applicability, a case study was investigated. The probabilistic integral risk was calculated in the chemical/petrochemical industrial area of Tarragona (Catalonia, Spain), where an environmental program has been carried out since 2002. The risk change between 2002 and 2005 was evaluated on the basis of probabilistic data of the levels of various pollutants in soils. The results indicated that the risk of the chemicals under study did not follow a homogeneous tendency. However, the current levels of pollution do not mean a relevant source of health risks for the local population. Moreover, the neuroprobabilistic HI seems to be an adequate tool to be taken into account in risk assessment processes.     

潜在客户资产测量研究

客户资产由当前客户资产和潜在客户资产两部分组成，对潜在客户资产测量问题尚未有系统的研究。在客户购买行为建模研究的基础上，提出由客户获取预测分析、客户购买行为分析和客户费用分析组成的潜在客户资产测量方法框架，总结了可用的模型，并用实证案例说明；提出利用当前潜在客户资产分析矩阵分析指导投资和管理决策的方法。     

Risk,trust and knowledge networks in farmers’ learning

This study reports on New Zealand dairy farmers’ access to and use of information as mediated through conditions of risk and trust within the context of their interpersonal social networks. We located participants’ reports of their information use within their perceived environments of trust and risk, following Giddens's [1990. The consequences of modernity. Polity Press, Stanford, CA] typology of trust and risk in pre-modernity and modernity. The research participants were constant users of interpersonal and print information from numerous sources, and monitored their incoming data in the light of strategic needs, reflecting their roles as both farming practitioners and business owners. Socio-spatial knowledge networks (SSKNs) combine individuals’ explanatory cognitive models of information acquisition and use with a micro-geographical analysis of their interpersonal networks. The participants showed characteristics of pre-modern, modern and even post-modern society in respect of their use of complex interactional forms, as well as a blending of individualistic and communitarian practices and concerns in their professional and personal lives.     

矩阵方程XA＝B的亚（半）正定自共轭四元数矩阵解

给出了四元数体Ｑ上ｎ×ｎ分块矩阵为亚（半）正定自共轭矩阵的一个充要条件，进而给出了Ｑ上矩阵方程ＸＡｎｍ＝Ｂｎｍ有亚（半）正定自共轭四元数矩阵解的充要条件及解集合的显式表示，从而推广改进了数城上线性方程组的反问题及矩阵反问题的相应结果．     

基于平衡计分卡的政府传统行政模式的转型

以传统政府模式转型成“企业化政府”模式为典型,把已经在企业内部取得瞩目成就的平衡计分卡管理模式引入“企业化政府”管理模式,增强政府改革的可行性和有效性。     

公民权与美国工业化时期缩短工时运动研究

在工业化时期,美国人们形成了"私人时间"的观念。他们普遍相信,过长的工时侵犯了自己的私人时间,而拥有私人时间是共和国公民的天然权利。这种思想成为缩短工时运动的重要思想基础,促进运动的蓬勃发展。然而,受契约自由传统的影响,运动仅取得了有限的成就,工人获得合理工作时间和私人时间的路途仍很艰辛漫长。     

平教会定县实验中的农村卫生工作之考察

中华平民教育促进会定县实验区在二十世纪三十年代创立了一套由村保健员、乡保健所、县保健院组成的三级卫生保健网,并在学校卫生、妇婴保健、传染病预防和疾病治疗等方面做出了显著的成绩,在当时产生了广泛的影响,对我们今天来说仍值得借鉴。