| 仙人掌茎多糖的分离纯化 |
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| 引用本文： | 罗显仲,陶美华,廖毅俊,陈燕,曾富华.仙人掌茎多糖的分离纯化[J].湛江师范学院学报,2004,25(6):32-36. |
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| 作者姓名： | 罗显仲 陶美华 廖毅俊 陈燕 曾富华 |
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| 作者单位： | 湛江师范学院,应用生命科学技术研究所,广东,湛江,524048 |
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| 基金项目： | 广东省科技厅科技攻关项目，广东省教育厅自然科学基金，湛江师院校科研和教改项目 |
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| 摘 要： | 采用水浸-醇沉法提取粗多糖,多糖得率为1.48%.仙人掌茎粗多糖再经DEAE-Sepharose fast flow柱层析,分离出3种多糖组分,3种多糖组分再经Sephadex-G 75和Sephadex-G200柱层析,得5种仙人掌茎多糖,均达到电泳纯,多糖的回收率在78.9%～95.8%之间.
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| 关 键 词： | 仙人掌 多糖 分离 纯化 |
| 文章编号： | 1006-4702(2004)06-0032-05 |
| 修稿时间： | 2004年7月11日 |
Isolation and Purification of Polysaccharides in the Cactus Pedicel |
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| LUO Xian - zhong,TAO Mei - hua,CHEN Yan,ZENG Fu - hua.Isolation and Purification of Polysaccharides in the Cactus Pedicel[J].Journal of Zhanjiang Normal College,2004,25(6):32-36. |
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| Authors: | LUO Xian - zhong TAO Mei - hua CHEN Yan ZENG Fu - hua |
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| Abstract: | The polysaccharide in the cactus was extracted by the water - soak and alcoholic - precipitation. The polysaccharides extracted from the cactus were isolated and purified through DEAE - Sepharose fast flow, and three kinds of polysaccharides were obtained. Further purification of the three kinds of polysaccharides was carried out through Sephadex - G 75 and Sephadex - G 200, and five kinds of polysaccharides were obtained. Their recovery was between 78.9 per cent and 95.8 per cent. |
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| Keywords: | cactus polysaccharide isolation purification |
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