硫酸镁对创伤性脑损伤大鼠血清NSE含量的影响

目的探讨硫酸镁对创伤性脑损伤大鼠血清神经元特异性烯醇化酶（neuron specific enolase,NSE）水平的影响。方法选用健康Sprague-Dawley（SD）大鼠,随机分为假损伤组、单纯脑损伤组、损伤给药组。然后制备自由落体脑损伤模型,损伤给药组大鼠在致伤后5分钟予25%硫酸镁（MgSO4）140mg/kg,腹腔注射,损伤24小时后分别测定脑组织含水量、放射免疫分析法测定大鼠血清NSE水平。结果与假损伤组比较,脑损伤24小时大鼠脑组织含水量明显增高（P〈0.01）,血清NSE水平升高（P〈0.05）。与单纯脑损伤组比较,损伤给药组可降低脑组织含水量（P〈0.05）,降低血清NSE水平（P〈0.05）。结论 MgSO4可以改善大鼠急性脑损伤后的脑水肿,降低血清NSE水平,改善创伤性脑损伤后继发性脑损害,具有神经保护作用。

The effection of magnesium sulfate on neuron specific enolase of traumatic brain injury in rats

Objective To explore the effect of magnesium sulfate on neuron specific enolase（ NSE） in traumatic brain injuried rats blood. Methods Healthy Sprague-Dawley（ SD） rats were randomly divided into magnesium sulfate administration group,traumatic brain injury but without drug administration group and sham-injury group. And then prepare the brain injury model of free-fall,administration of magnesium sulfate at a dose of 140 mg /kg,intraperitoneal injection after the injury,24 hours after the traumatic brain injury,the brain edemas was evaluated,and expression of NSE level was determined in rats blood by radioimmunoassay method. Results Compared with sham-injury group,24 hours after traumatic brain injury,the brain edemas of rats increase（ P 〈 0. 05）,NSE level increase in blood（ P 〈 0. 05）. Compared with the group with traumatic brain injury but without drug administration,magnesium sulfate pretreatment group improved the brain edemas of rats（ P 〈 0. 05）,and reduce NSE level in the rats blood（ P〈 0. 05）. Conclusion MgSO4 can decrease the brain edemas of traumatic brain injury rats,lower NSE level in brain blood,MgSO4 has neuroprotective effect against traumatic brain injury in rats.