正交试验优选马铃薯糖苷生物碱提取工艺

对马铃薯中糖苷生物碱的超声提取工艺进行了研究。以α-茄碱为对照品,通过L9（34）正交试验,优选了工艺参数。结果表明：选用含5%醋酸的乙醇为提取溶剂,各因素的影响依次为：提取次数〉乙醇体积分数〉料液比〉提取时间。最优提取工艺条件是：以60%的乙醇为溶剂,提取时间30 min,料液比1：10,三级提取,粗提物得率薯芽最高为0.016g/g,测得粗提物糖苷生物碱含量230 mg/g;薯芽、薯皮、薯肉中糖苷生物碱含量分别为3.37 mg/g、2.91 mg/g、0.68 mg/g。

Study on the Extraction Technology of Glycoalkaloids Contents in Potato by Orthogonal Design

Ultrasonic assisted extraction technology of glycoalkaloids in the potato was studied,and the optimal conditions were chosen through L9（34） orthographic experiments based on a-solanine as standard substance.The result indicated the effect order of four factors affecting extractor productivity were as follows： extracting times volume percentage of ethonal ratio of material to liquid time,and when the extraction parameters are composed of three times,60%,1：10 ratio of material and 30 minutes to liquid,the yields of glycoalkaloids are maximum which extraction productivity is 0.016 g/g（bud of potato）,and content of total glycoalkaloids is 230 mg/g.besides the study showed glycoalkaloids in bud,tegument and mass of potato were 3.37 mg/g、2.91 mg/g、0.68 mg/g respectively.