Tolerance‐based error reduction in multi‐agent bioassays

Some degree of error is inevitable in multi‐agent bioassays regardless of design or measurement technology. Estimation error can be reduced post facto by exploiting the matrix partial ordering of the bioassay survival estimates. The standard method for this is order‐restricted regression (ORR). If the joint action of the bioassay agents admits a tolerance‐based interpretation, additional structure beyond matrix partial ordering is available, leading to a new method of error reduction. This tolerance‐based error reduction (TBER) procedure almost always outperforms ORR. Like ORR, TBER applies to complete factorial bioassay designs and, using weighting, to incomplete designs.