| 新金属菌绿素对HL-60肿瘤细胞光毒作用的参数研究 |
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| 引用本文： | 胡锐.新金属菌绿素对HL-60肿瘤细胞光毒作用的参数研究[J].琼州学院学报,2010,17(2):30-33. |
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| 作者姓名： | 胡锐 |
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| 作者单位： | 琼州学院理工学院,海南,五指山,572200 |
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| 摘 要： | 细菌叶绿素锌作为一类潜在的长波长光敏剂,具有重要生物医学研究价值.采用MTT法研究Zn-BCA-PDT灭杀HL-60细胞的浓度、暗孵育时间、照光时间和光波长等参数,总结对HL-60白血病肿瘤细胞进行Zn-BCA-PDT的较佳处理方案：细胞密度1×105个/mL,Zn-BCA浓度10μg/mL,暗室孵育3 h,照光时间50 min,光源波长为800 nm.
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| 关 键 词： | 细菌叶绿素锌 HL-60 MTT 细胞光毒作用 Photodynamic treat（PDT） |
Research of Parameters on New Type Long Wavelength Photosensitizer Destruction of Leukaemie cell HL-60 |
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| HU Rui.Research of Parameters on New Type Long Wavelength Photosensitizer Destruction of Leukaemie cell HL-60[J].Journal of Qiongzhou University,2010,17(2):30-33. |
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| Authors: | HU Rui |
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| Institution: | HU Rui (College of Science , Engineering,Qiongzhou University,Wuzhishan Hainan 572200,China) |
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| Abstract: | This article describes the work of Zn-BCA-PDT to HL-60 cell,which includes parameters of Zn-BCA density,culture time,illumination time and wavelength of light,and summarizes the optimized method of experiment using Zn-BCA-PDT for the HL-60 cell： cell density is 1×105/mL,Zn-BCA density is 10 μg/mL,incubation time with Zn-BCA is 3 h,light wavelength is 800 nm. |
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| Keywords: | zinc-bacteriochlorophyll HL-60 MTT light poison of cell PDT |
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