| 鹅血SOD的纯化与鉴定 |
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| 引用本文： | 李竞,肖昌,张耕,鞠静丽,许南萍.鹅血SOD的纯化与鉴定[J].西南农业大学学报(社会科学版),2002(1). |
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| 作者姓名： | 李竞 肖昌 张耕 鞠静丽 许南萍 |
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| 作者单位： | 西南农业大学荣昌校区生物技术系 重庆荣昌402460
(李竞，肖昌，张耕，鞠静丽)，西南农业大学荣昌校区生物技术系 重庆荣昌402460(许南萍) |
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| 摘 要： | 新鲜鹅血经氯仿—乙醇分级分离 ,丙酮沉淀及DEAE纤维素层析的方法得到纯铜锌超氧化物歧化酶 (Cu .Zn -SOD)。每升鹅血可获得纯化的SOD总活力为 2 314 0 0U ,比活力为 7713U/mg。经聚丙烯酰胺凝胶电泳后显示出两条带。结果表明用该纯化方法得到的SOD为均一性纯酶
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| 关 键 词： | 鹅血 SOD 纯化 鉴定 |
PURIFICATION AND IDENTIFICATION OF A SUPEROXIDE DISMUTASE FROM GOOSE BLOOD |
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| Li Jing,Xiao Chang,Zhang Geng,et al..PURIFICATION AND IDENTIFICATION OF A SUPEROXIDE DISMUTASE FROM GOOSE BLOOD[J].Journal of Southwest Agricultural University:Social Science Edition,2002(1). |
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| Authors: | Li Jing Xiao Chang Zhang Geng |
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| Institution: | Li Jing Xiao Chang Zhang Geng et al. |
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| Abstract: | Superoxide dismutase(SOD) from fresh goose blood was purified by Chloroform-ethanol fractionation, acetone sedimentation and DEAE-cellulose chromatography. The specific activity of the purified enzyme is 7 713 U/mg. The enzyme shows two bands in SDS PAGE. The results show that the SOD purified by this method is homogenously pure. |
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| Keywords: | Superoxide dismutase Goose blood Purification Identification |
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