白藜芦醇对耐力训练小鼠抗氧化能力的影响

研究白藜芦醇对耐力训练小鼠抗氧化能力和线粒体游离钙含量的影响．选用昆明小鼠，根据体重将72只小鼠随机分为三组：A组为安静对照组；B组为耐力训练组；C组为耐力训练＋白藜芦醇组．C组小鼠每日给予白藜芦醇10mg／kg灌胃，与B组共同进行6w递增负荷游泳训练，再进行力竭游泳实验．力竭游泳后24h处死小鼠取材，测定小鼠骨骼肌、心脏、胸腺，脾脏、肾脏、肝脏线粒体和红细胞SOD、GSH-Px活性及MDA含量。以及肝脏线粒体游离钙的含量．6w递增负荷耐力训练后，B组小鼠骨骼肌、心肌、肝脏线粒体、脾脏、胸腺和红细胞SOD活性，心肌、脾脏和红细胞的GSH—Px活性均显著低于A组（P〈0．05），同时骨骼肌、肝脏线粒体、脾脏和红细胞的MDA含量显著高于A组（P〈0．05）。肝脏线粒体游离钙含量显著高于A组（P〈0．05）；白藜芦醇干预使C组小鼠骨骼肌、肝脏线粒体和脾脏SOD活性，骨骼肌和心肌的GSH—Px活性显著高于B组（P〈0．05），同时骨骼肌、肝脏线粒体、胸腺和红细胞的MDA含量显著低于B组（P〈0．05）；肝脏线粒体游离钙含量显著低于B组（P〈0．05）．提示白藜芦醇一定程度上能提高耐力训练小鼠抗氧化能力，维持线粒体游离钙含量在正常水平，具有延缓运动性疲劳发生的作用．

Effects of Resveratrol on Anti——oxidation capacity of Mice Performing Endurance Exercise

Objective The objective of this study is to investigate the effects of resveratrol on anti-oxidation and Ca^2＋ content in liver mitochondria of mice performing endurance exercise. Methods Kunming mice, 72, were randomly divided into 3 groups according to weight： group A （Sedentary Control）, group B （Endurance exercise）, and group C （Resveratrol ＋ Endurance exercise）. Administrated of resveratrol with 10 mg kg^-1d^-1 , the mice of group C were taken incremental load protocol of swimming for six weeks, and then had a exhaustion swimming as did the mice of group B. All mice were sacrificed and sampled 24 h later. The following parameters in mice were tested. SOD activity, GSH-Px activity and MDA content in skeletal muscle, cardiac muscle, thymus, spleens, kidney, liver mitochondria and erythrocytes, Ca^2＋ content in liver mitochondria. Results SOD activity in skeletal muscle, cardiac muscle, liver mitochon dria, spleens, thymus and erythrocytes, GSH-Px activity in cardiac muscle, spleens and erythrocytes in training mice were lower than that in sedentary control group after 6 weeks training （ P 〈0.05）, while MDA content in skeletal muscle, liver mitochondria, spleens and erythrocytes, and and contain Ca^2＋ content in liver mitochondria significantly increased compared with sedentary control group （ P 〈0.05）. However, SOD activity in skeletal muscle, liver mitochondria and spleens, and GSH-Px activity in skeletal muscle and cardiac muscle significantly increased （ P 〈0.05）, MDA content in skeletal muscle, liver mitochondria, thymus and erythrocytes significantly decreased （ P 〈0.05）, and Ca^2＋ content in liver mitochondria also significantly decreased in resveratrol treatment group mice compared with endurance mice （P 〈 0. 05）. Therefore, it was suggested that reseveratrol increased the anti-oxidation capacity of mice performing endurance exercise and keep Ca^2＋ content in liver mitochondria at normal level, and thus delayed exercise-induced fatigue.