| 基于生长时间光谱法的大肠菌群单管定量检测技术研究 |
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| 引用本文： | 蔡尽忠,庄峙厦,赵丽,汤新华,鄢庆枇.基于生长时间光谱法的大肠菌群单管定量检测技术研究[J].福建农林大学学报(哲学社会科学版),2011(5). |
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| 作者姓名： | 蔡尽忠 庄峙厦 赵丽 汤新华 鄢庆枇 |
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| 作者单位： | 厦门华厦职业学院食品药品安全检测实验室;厦门大学化工学院;厦门斯坦道生物公司;集美大学水产学院; |
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| 基金项目： | 厦门市科技计划创新项目(3502Z20103046) |
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| 摘 要： | 通过研究大肠菌群初始接种浓度与达到0.2吸光度所需时间的关系,建立基于生长时间光谱法的大肠菌群单管定量检测技术。通过与多管发酵法比较发现单管定量检测技术的相对标准偏差小于传统的多管发酵法,而且反应所需的时间短,当初始菌浓为101.3cfu/mL时反应时间仅需9h,可以作为大肠菌群快速检测的方法,具有良好的应用前景。
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| 关 键 词： | 大肠菌群 ONPG 生长时间光谱法 单管定量检测 |
Establishment of Single Tube Quantitative Detection Technology for Coliforms Assay Based on Time of Growth Spectrophotometer Method |
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| CAI Jin-zhong,ZHUANG Zhi-xia,ZHAO Li,TANG Xin-hua,YAN Qing-Pi.Establishment of Single Tube Quantitative Detection Technology for Coliforms Assay Based on Time of Growth Spectrophotometer Method[J].Journal of Fujian Agriculture and Forestry University,2011(5). |
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| Authors: | CAI Jin-zhong ZHUANG Zhi-xia ZHAO Li TANG Xin-hua YAN Qing-Pi |
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| Institution: | CAI Jin-zhong1,ZHUANG Zhi-xia1,2,ZHAO Li1,TANG Xin-hua3,YAN Qing-Pi4(1.Laboratory of Food and Drug Safety Test,Xiamen Huaxia Vocational College,Xiamen,Fujian 361024)(2.College of ChemicalEngineering,Xiamen University,Fujian 361005)(3.Xiamen Standard Biotechmology Company,Fujian 361006)(4.Fisheries College of Jimei University,Fujian 361021) |
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| Abstract: | By studying the initial inoculation concentration of coliforms and the time required to reach 0.2 absorbance,and establishment of single tube quantitative detection technology for coliforms assay based on time of growthspectrophotometer method.By comparison with the multi-tube fermentation method of quantitative detection of single-tube technology that the relative standard deviation less than the conventional multi-tube fermentation method,and the reaction time is short,when the initial bacterial concentra... |
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| Keywords: | coliforms ONPG time of growth spectrophotometer method single-tube quantitative detection |
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