| 微量热法在酶促反应热动力学性质研究中的应用 |
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| 引用本文： | 谢修银.微量热法在酶促反应热动力学性质研究中的应用[J].长江大学学报(社会科学版),1996(5). |
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| 作者姓名： | 谢修银 |
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| 摘 要： | 讨论了用微量热法测定酶促反应的米氏常数，酶活性，表观速率常数及表观活化能等动力学参数的实验方法和测定原理．介绍了摩尔反应焓的测定和计算方法，并借助过渡状态理论，探讨了酶底复合过程的吉氏自由能、焓和熵等热力学函数值的测定和计算方法
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| 关 键 词： | 微量热法 酶促反应 热力学性质 动力学性质 |
APPLICATION OF MICROCALORIMETRY FOR STUDYINGTHE THERMODYNAMIC AND KINETIC PROPERTIESOF ENZYMIC REACTION |
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| Xie Xiuyin.APPLICATION OF MICROCALORIMETRY FOR STUDYINGTHE THERMODYNAMIC AND KINETIC PROPERTIESOF ENZYMIC REACTION[J].Journal of Yangtze University:Social Sciences,1996(5). |
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| Authors: | Xie Xiuyin |
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| Institution: | Department of Chemistry |
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| Abstract: | Determination of the kinetic properties of enzymic reaction,sueh as Michaelis constant,enzyme activity,observed velocity constant and activation energy with microcalorimetry have been discussed The total molar enthalpy of enzymic reaction,the activation free energy,activation enthalpy,and activation entropy of the composite process between enzyme and substrate are studied with microcalori metry in this thesis |
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| Keywords: | microcalorimetry enzymic reaction thermodynamic propeoty kinetic property |
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