| 荞麦组织培养中的植株再生 |
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| 引用本文： | 陈发菊,陈芳清,谭立槐.荞麦组织培养中的植株再生[J].三峡大学学报(人文社会科学版),2000(2). |
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| 作者姓名： | 陈发菊 陈芳清 谭立槐 |
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| 作者单位： | 湖北三峡学院医学院生物系!湖北省宜昌443003(陈发菊，陈芳清)，湖北省巴东县水利局!湖北巴东444300(谭立槐) |
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| 摘 要： | 用荞麦无菌苗的幼茎和幼叶作外植体分别接种在MS补加不同激素组合的培养基上培养获得再生植株 实验结果表明 ,适宜诱导愈伤组织的激素组合为 2 4-D 4 0mg/L KT0 2mg/L ,适宜愈伤组织生长的激素组合为 2 4-D 1 0mg/L KT 1 0mg/L 诱导芽的较适宜的组合为 6 -BA 3 0mg/L IAA 0 1mg/L 不定芽接种到含MS IBA 2 0mg/L KT 0 1mg/L的MS培养基上就能生根
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| 关 键 词： | 荞麦 愈伤组织 器官发生 再生植株 |
Tissue Culture and Plant Regeneration of Buctwheat (Fagopyren esculentum Moench) |
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| CHEN Fa_ju ,CHEN Fang_qing ,TAN Li_huai.Tissue Culture and Plant Regeneration of Buctwheat (Fagopyren esculentum Moench)[J].Journal of China Three Gorges University(Humanities & Social Sciences),2000(2). |
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| Authors: | CHEN Fa_ju CHEN Fang_qing TAN Li_huai |
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| Institution: | CHEN Fa_ju 1,CHEN Fang_qing 1,TAN Li_huai 2 |
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| Abstract: | Stem segments and leaf segments of Buckwheat were cultured on MS medium supplemented with different phytohormone compositions and the plant regrneration was obtained The experiment's results showed that 2 4-D was necessary for inducting callus of buckwheat The suitable phytohormone composition to induct callus was 2 4-D 4 0mg/L KT 0 2mg/L The suitable phytohormone compsition for callus growing was 2 4-D 1 0mg/L KT 1 0mg/L; It was very difficult to induce the adventitious buds from the callus kept on 2 4-D KT medium The suitable medium to induce adventitious buds and roots were 6-BA 3 0mg/L IAA 0 1mg/L MS and IBA 2 0mg/L KT 0 1 mg/L MS respectively |
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| Keywords: | Buckwheat Callus Organogenesis Plant regeneration |
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