子宫内膜癌EphB4mRNA和EphrinB2 mRNA的表达及临床意义

目的研究EphB4mRNA和Ephrin B2mRNA在子宫内膜癌中的表达及其意义。方法应用逆转录-聚合酶链式反应（RT-PCR）分别检测经免疫组化证实有不同级别的EphB4和Ephrin B2蛋白表达的35例子宫内膜癌冰冻标本中的mRNA水平。比较各自与其蛋白表达的相关性及与临床病理因素的关系。结果子宫内膜癌组织中EphB4mRNA和Ephrin B2mRNA的表达均与组织学分级、淋巴结转移及临床分期均显著相关（P〈0.05）.EphB4和Ephrin B2蛋白表达和mRNA表达水平不完全一致。结论 EphB4、Ephrin B与子宫内膜癌的发生发展有关。其联合检测有助于客观评估子宫内膜癌的生物学行为,可用来指导临床用药和预测预后。

The expressions of EphB4mRNA and EphrinB2mRNA in endometrical carcinoma and their significance

Objective To study the expressions of EphB4mRNA,EphrinB2mRNA in endometrical carcinomas and to explore their significance.Methods The levels of EphB4 and EphrinB2 mRNA in frozen tissues of 35 endometrical carcinoma cases in which the tumours showed different grades were detected by RT-PCR and compared if the levels of mRNA correspond to their levels of protein and clinicopathological features.Results The positive expressions of EphB4mRNA and EphrinB2mRNA were both associ-ated with histological grade,lymph node metastasis and clinical stage respectively（P〈0.05）.The expression of EphB4mRNA and EphrinB2 did not fully correspond to their protein expression in endometrical carcinoma.Conclusions EphB4,EphrinB2 may be in-volved in carcinogenesis,development procedures of endometrical carcinoma.The combined detection of them can help to predict the clinical and pathologic characteristics of endometrial carcinoma and to guide treatment and tell the progonosis.