Wild bee abundance declines with urban warming,regardless of floral density

As cities expand, conservation of beneficial insects is essential to maintaining robust urban ecosystem services such as pollination. Urban warming alters insect physiology, fitness, and abundance, but the effect of urban warming on pollinator communities has not been investigated. We sampled bees at 18 sites encompassing an urban warming mosaic within Raleigh, NC, USA. We quantified habitat variables at all sites by measuring air temperature, percent impervious surface (on local and landscape scales), floral density, and floral diversity. We tested the hypothesis that urban bee community structure depends on temperature. We also conducted model selection to determine whether temperature was among the most important predictors of urban bee community structure. Finally, we asked whether bee responses to temperature or impervious surface depended on bee functional traits. Bee abundance declined by about 41% per °C urban warming, and temperature was among the best predictors of bee abundance and community composition. Local impervious surface and floral density were also important predictors of bee abundance, although only large bees appeared to benefit from high floral density. Bee species richness increased with floral density regardless of bee size, and bee responses to urban habitat variables were independent of other life-history traits. Although we document benefits of high floral density, simply adding flowers to otherwise hot, impervious sites is unlikely to restore the entire urban pollinator community since floral resources benefit large bees more than small bees.     

Relationship between land cover and insectivorous bat activity in an urban landscape

Despite the clear importance of conserving biodiversity in urbanized areas, research on how bats are influenced by urbanization has is only recently catching up to the more established body of research for urban birds. Much of the extant research has been limited to urban parks and other natural areas. Here I present the results of an acoustic survey of bats throughout the agricultural/forest/suburban/urban mosaic of the Minneapolis-St. Paul metropolitan area. Activity indices by each of the six recorded species (Eptesicus fuscus, Lasiurus cinereus, Lasiurus borealis, Lasionycteris noctivagans, Myotis sp., and Perimyotis subflavus) were used as response variables with land cover variables in a series of statistical models. Because bats may perceive the landscape at different scales, candidate models included land cover variables within 100, 500 and 1,000?m of the sampling location. Activity of L. noctivagans was negatively related to the amount of impervious surface and open habitat in the immediate landscape. L. borealis activity was positively correlated with immediate tree cover and with impervious surface at the 1,000?m scale. Tree cover and proximity to water were positively related to activity levels of Myotis sp., L. cinereus and P. subflavus, but only the latter was influenced by the amount of surrounding impervious surface. These results suggest that a one-size fits all approach to bat conservation in human modified landscapes is not appropriate, but also that certain species appear to do well in urban environments provided that there is sufficient tree cover.     

Does urbanization explain differences in interactions between an insect herbivore and its natural enemies and mutualists?

Urbanization can alter the composition of arthropod communities. However, little is known about how urbanization affects ecological interactions. Using experimental colonies of the black bean aphid Aphis fabae Scopoli reared on Vicia faba L, we asked if patterns of predator-prey, host-parasitoid and ant-aphid mutualisms varied along an urbanization gradient across a large town in southern England. We recorded the presence of naturally occurring predators, parasitoid wasps and mutualistic ants together with aphid abundance. We examined how biotic (green areas and plant richness) and abiotic features (impervious surfaces and distance to town center) affected (1) aphid colony size, (2) the likelihood of finding predators, mutualistic ants and aphid mummies (indicating the presence of parasitoids), and (3) how the interplay among these factors affected patterns of parasitoid attack, predator abundance, mutualistic interactions and aphid abundance. Aphid abundance was best explained by the number of mutualistic ants attending the colonies. Aphid predators responded negatively to both the proportion of impervious surfaces and to the number of mutualistic ants farming the colonies, and positively to aphid population size, whereas parasitized aphids were found in colonies with higher numbers of aphids and ants. The number of mutualistic ants attending was positively associated with aphid colony size and negatively with the number of aphid predators. Our findings suggest that for insect-natural enemy interactions, urbanization may affect some groups, while not influencing others, and that local effects (mutualists, host plant presence) will also be key determinants of how urban ecological communities are formed.     

Both semi-natural and ruderal habitats matter for supporting insect functional diversity in an abandoned quarry in the city of Kraków (S Poland)

Urban areas are beginning to be recognized as having potential for biodiversity conservation because of the relatively high richness of some taxa. However, little is known about what functional groups of organisms constitute this richness. We investigated biodiversity patterns in an abandoned quarry using the Rapid Biodiversity Assessment method based on insect groups according to their trophic guilds. We assessed the value of semi-natural and ruderal habitats. The ruderal sites were characterized by a higher diversity and abundance of phytophagous beetles and, unexpectedly, of parasitoids and predatory beetles, whereas the reverse was true for Hemiptera and Aculeata. Patterns of α-diversity were impacted by different factors than β-diversity: these primarily acted in opposite directions, depending on the habitat type. Species richness was positively related to the woody surroundings of habitat patches on semi-natural sites, but negatively related on ruderal sites. The Dominance index was negatively affected by human impact. Insect assemblages were diversified by taller vegetation, higher nutrient content, lesser human impact and a lower level of insolation in the grasslands with ruderal vegetation than in the semi-natural grasslands. A particular habitat type may constitute a source for some insect groups but a sink for others. Ruderal habitats utilized as a substitute for loss of the semi-natural vegetation are essential for the preservation of insect functional diversity and are suitable for vulnerable groups such as Parasitica. Post-industrial areas with a habitat mosaic of semi-natural and ruderal sites may enrich biodiversity in the urban landscape.

     

Insects on urban plants: contrasting the flower head feeding assemblages on native and exotic hosts

Exotic plant species very often comprise a large proportion of urban floras. Because herbivorous insects depend on the presence of suitable host plants to maintain their populations, it is imperative to elucidate the relative importance of native and exotic hosts to understand the response of herbivorous guilds to urbanization. By using a plant-herbivore system composed of Asteraceae hosts and flower-head endophagous insects, we investigated whether the diversity and composition of herbivorous insects differs between native and exotic host-plant species in an urban environment. Although we found only seven exotic Asteraceae among the 30 species recorded, the overall abundance of exotics was considerably greater than that of native host plants. Overall, the exotic host species supported a small subset of the herbivore assemblage found on the native ones. The number of herbivore species per host species was significantly higher among the native plants, but we did not find a difference in herbivore abundance. Moreover, the higher taxonomic composition of herbivores on exotic Asteraceae was reduced, being composed of only three genera and two families from a total of 16 genera and six families of herbivores. These results provide support for the idea that plants outside of their original geographic distribution have lower loads of enemies than phylogenetically related native species. Our findings indicate that native host plants in urban areas play a critical role in supporting the native herbivorous insect fauna.     

Patterns of pollinator turnover and increasing diversity associated with urban habitats

The role of urban expansion on bee diversity is poorly understood, but it may play an important role in restructuring pollinator diversity observed in rural regions at the urban perimeter. We studied bee communities in two habitats essential for pollinators (residential gardens and semi-natural areas) at 42 sites situated at the edge of greater Montreal, Canada. Bee species richness, abundance and functional diversity all increased with urbanization in both habitat types, but gardens and semi-natural areas supported distinct bee communities with unique responses to urbanization in terms of species turnover. Compared to semi-natural sites, residential gardens supported bees that foraged from a greater number but a lower proportion of available plant species. Bees did not discriminate between exotic and indigenous plant species in either gardens or semi-natural sites and were attracted to flowers in either habitat irrespective of their origins. Protecting semi-natural ruderal areas and providing residential garden habitats for pollinators are both effective means of promoting regional bee diversity in urbanizing regions.     

Silphids in urban forests: Diversity and function

Many ecologists have examined the process of how urbanization reduces biological diversity but rarely have its ecological consequences been assessed. We studied forest-dwelling burying beetles (Coleoptera: Silphidae)—a guild of insects that requires carrion to complete their life cycles—along an urban-rural gradient of land use in Maryland. Our objective was to determine how forest fragmentation associated with urbanization affects (1) beetle community diversity and structure and (2) the ecological function provided by these insects, that is, decomposition of vertebrate carcasses. Forest fragmentation strongly reduced burying beetle diversity and abundance, and did so far more pervasively than urbanization of the surrounding landscape. The likelihood that beetles interred experimental baits was a direct, positive function of burying beetle diversity. We conclude that loss of burying beetle diversity resulting from forest fragmentation could have important ecological consequences in urban forests.     

Assessment of landscape patterns affecting land surface temperature in different biophysical gradients in Shenzhen, China

The urban heat island (UHI) effect is one of the important ecological effects of urbanization. This study focuses on the different effects of landscape patterns on LST within different land covers. The land cover was measured by surface biophysical components, including vegetation fraction (VF) and impervious surface area (ISA), acquired by a linear spectral mixture model (LSMM). LST was derived from Landsat-5 TM thermal infrared (TIR) data using the generalized single-channel method. Landscape patterns were measured by landscape metrics, including the Shannon diversity index (SHDI), the aggregation index (AI), patch density (PD), and fractal dimension area-weighted mean index (FRAC_AM). Shenzhen, a rapidly urbanizing city in China, was taken as the case study area. Results showed that VF and ISA are more important than spatial patterns in determining LST. However, these effects change in densely covered areas. VF and LST are negatively correlated, with the inflection of the regression curves being 45 %. In areas with VF lower than 45 %, the correlation between LST and VF is monotonically linear. In areas with VF higher than 45 %, landscape patterns can act to decrease LST. The aggregation index (AI) and the largest patch index (LPI) can contribute to decreasing LST significantly. Impervious surfaces contribute to high temperature, and the inflection point of the regression curves is 70 %. In areas with ISA higher than 70 %, a fragmented pattern of impervious surfaces can lower LST. These findings provide insights for planners into how the strategic use of landscape to mitigate UHI effects may vary for different land covers.     

Assessing effect of dominant land-cover types and pattern on urban forest biomass estimated using LiDAR metrics

Accurate estimates of biomass in urban forests can help improve strategies for enhancing ecosystem services. Landscape heterogeneity, such as land-cover types and their spatial arrangements, greatly affects biomass growth, and it complicates the estimation of biomass. Application of LiDAR data is a typical approach for mapping forest biomass and carbon stocks across heterogeneous landscapes. However, little is known about how urban land uses and pattern impact biomass and estimates derived from LiDAR analysis. In this study, we examined the relationship between LiDAR-derived biomass and dominant land-cover types using field-measured estimates of aboveground forest biomass in an urbanized region of North Carolina, USA. Three objectives drove this research: 1) we examined the local effects of dominant land cover types on urban forest biomass; 2) we identified the spatial scale at which dominant land cover influences biomass estimates; 3) we investigated whether the fine-scale, spatial heterogeneity of the urban landscape contributed to forest biomass. We used multiple linear regression to relate field-measured biomass to LiDAR metrics and land cover densities derived from Landsat and LiDAR data. The biomass model developed from variables derived from LiDAR first returns produced biomass estimates similar to using all LiDAR returns. Although three land-cover types (impervious surface, managed clearings, and farmland) exhibited a negative relationship with biomass, only impervious surface was statistically significant. The biomass model that used impervious surface densities between 100 m and 175 m radial buffers produced the highest adjusted R ² with lower RMSE values. Our study suggests that impervious surface impacted forest biomass estimates considerably in urbanizing landscapes with the greatest effect between 100 and 175 m from a forest stand. Managed clearing and farmland types negatively impacted biomass estimation albeit not as strongly as impervious surface. Overall, we found that accounting for impervious surface density and its proximity to forest in biomass models may improve urban forest biomass estimates.     

Common factors influence bee foraging in urban and wildland landscapes

Bees are important flower-visiting insects that display differential occurrences at food resources throughout urban and wildland landscapes. This study examined the visitation rates and foraging patterns of eight taxonomic groups of bees that are common to California poppies, Eschscholzia californica, in both landscape types. Bee occurrence was documented in relation to floral resource characteristics (patch area, poppy coverage, and poppy density), local landscape characteristics (distance to the wildland-urban interface, distance to riparian areas, distance to green space, and land use), and regional landscape context (urban versus wildland). Similar abundance and richness measures were recorded at both urban and wildland poppy patches, but community composition varied in each landscape. Bumble bees were more abundant at poppies in the wildland whereas species in the family Halictidae (sweat bees) were more abundant at poppies within the urban landscape. Resource patch size and density consistently correlated with increased bee presence for all bee types foraging in the wildland. Individual patterns of occurrence in the urban landscape were somewhat divergent; the foraging dynamics of larger bodied-bees (Bombus vosnesenskii and Megachile species) correlated significantly with resource patch size and density, whereas smaller-bodied bees (family Halictidae and Andrena species) were influenced by landscape characteristics such as distance to the wildland-urban interface and distance to riparian areas. In summary, the surrounding landscape had an influence on community composition, but the magnitude of the floral resource present at a site and factors relating to foraging energetics were dominant drivers of local occurrence. These results suggest that management strategies that provide dense and abundant floral resources should be successful in attracting bees, irrespective of their location within the urban matrix.     

Relation of urbanization to stream fish assemblages and species traits in nine metropolitan areas of the United States

We examined associations of fish assemblages and fish traits with urbanization and selected environmental variables in nine major United States metropolitan areas. The strongest relations between fishes and urbanization occurred in the metropolitan areas of Atlanta, Georgia; Birmingham, Alabama; Boston, Massachusetts; and Portland, Oregon. In these areas, environmental variables with strong associations (r_s ≥ 0.70) with fish assemblages and fish traits tended to have strong associations with urbanization. Relations of urbanization with fish assemblages and fish traits were weaker in Denver, Colorado; Dallas-Fort Worth, Texas; Milwaukee-Green Bay, Wisconsin; and Raleigh, North Carolina. Environmental variables associated with fishes varied among the metropolitan areas. The metropolitan areas with poor relations may have had a limited range of possible response because of previous landscape disturbances. Given the complexities of urban landscapes in different metropolitan areas, our results indicate that caution is warranted when generalizing about biological responses to urbanization.     

Species change in an urban setting—ground and rove beetles (Coleoptera: Carabidae and Staphylinidae) in Berlin

The influence of environmental parameters on epigeic beetle communities of forest fragments in an urbanization gradient was studied in Berlin. Eight deciduous forests along a rural to urban gradient were sampled with pitfall traps. Species richness did not decline across the rural to urban gradient. As expected, impervious surface cover as an indicator of urbanization correlated not only with habitat fragmentation and heat island effect but also with altered soil properties. The proportion of forest specialist staphylinid species decreased with increasing urbanization. The differences between staphylinid communities of neighboring forest fragments were enhanced in the most urban parts, probably due to increased habitat fragmentation. Furthermore, the loss of flightless species with increasing habitat isolation emphasized the influence of habitat fragmentation. The carabid communities revealed the urbanization effects not as clearly as the staphylinid communities, but both taxa revealed that direct anthropogenic habitat alteration, indicated by removal of decaying wood, favors open-habitat specialists. The extent of the urbanization influence seems to vary seasonally. Environmental parameters associated with urbanization explain the ordination of species communities in the winter better than in the summer. Heat island effect is suggested as an explanation for this difference.     

Field and remotely sensed measures of soil and vegetation carbon and nitrogen across an urbanization gradient in the Boston metropolitan area

Understanding the impact of urbanization on terrestrial biogeochemistry is critical for addressing society’s grand challenge of global environmental change. We used field observations and remotely sensed data to quantify the effects of urbanization on vegetation and soils across a 100-km urbanization gradient extending from Boston to Harvard Forest and Worcester, MA. At the field-plot scale, the normalized difference vegetation index (NDVI) was positively correlated with aboveground biomass (AGB) and foliar nitrogen (N) content and negatively correlated with impervious surface fraction. Unlike previous studies, we found no significant relationship between NDVI or impervious surface area (ISA) fraction and foliar N concentration. Patterns in foliar N appeared to be driven more strongly by changes in species composition rather than phenotypic plasticity across the urbanization gradient. For forest and non-residential development, soil nitrogen content increased with urban intensity. In contrast, residential land had consistently high soil N content across the gradient of urbanization. When field observations were scaled-up to the Boston Metropolitan Statistical Area (MSA), we found that soil and vegetation N content were negatively correlated with ISA fraction, an indicator of urban intensity. Our results demonstrated the importance of accounting for the influence of impervious surfaces when scaling field data across urban ecosystems. The combination of field data with remote sensing holds promise for disentangling the complex interactions that drive biogeochemical cycling in urbanizing landscapes. Empirical data that accurately characterize variations in urban biogeochemistry are critical to gain a mechanistic understanding of urban ecosystem function and to guide policy makers and planners in developing ecologically sensitive development strategies.     

The effects of landscape scale on greenery and traffic relationships with urban birds and butterflies

Actions and policies to enhance biodiversity in the urban landscape must match the spatial scale at which biodiversity responds to the management and target variables. To this end, we compare the importance and effect of different kinds of greenery cover and road-lane density on bird and butterfly species richness between two landscape scales: 50-m versus 126-m radii around point counts (equivalent to areas of 0.8 h and 5 ha, respectively). We also compared the results against those of an earlier study using 500-m walking transects with widths of 100 m (i.e., 5 ha). Road lane density was more important at the 126-m than 50-m radius for both birds and butterflies. For birds, natural vegetation or forest cover and cultivated shrub cover were also more important at 126-m radius whereas the cultivated tree canopy cover was more important at 50-m radius. Cultivated tree cover and natural vegetation or forest cover were positively associated with species richness while road lane density and cultivated shrub cover were negatively associated with species richness. The results from point counts generally corroborate the results from the transects-based study, except that the short-duration point counts performed poorly in sampling butterflies. Our results indicate that in designing urban greenery policy, the plot sizes of individual developments is an appropriate spatial scale for the stipulation of tree cover targets, while urban planners have more flexibility to allocate natural greenery at broader spatial scales.

     

Bird responses to housing development in intensively managed agricultural landscapes

Housing a growing human population is a global issue and designing environmentally friendly developments requires identifying the species likely to be negatively impacted and finding mitigation solutions. Existing studies that consider fragmentation of natural habitats have limited application in countries such as Britain where a prime target for development is agricultural land where decades of intensive management have already diminished biodiversity. Here I used citizen science data on the abundance of 146 breeding and wintering birds to develop models linking abundance to human population density and habitat features. I used these as a proxy for the urbanisation process, finding that impacts of urbanisation were species-specific and context dependent. Low-density developments benefited a high proportion of birds, with wetland birds benefitting most and farmland birds least, but as human densities increased further, up to 75% of species were negatively impacted. Almost half of species currently occurring at 14 flagship residential development sites were predicted to decline based on projected human population density increases, with a third predicted to increase. Presence of wetlands, canopy cover and patches of trees all benefited certain species but efforts to identify more detailed habitat associations were hampered by collinearity among variables. I conclude that even in heavily degraded agricultural landscapes, a high proportion of species will be negatively impacted by residential development and that some will require spared land to persist in the wider landscape. As no single habitat benefited the entire bird community, urban planners wishing to design bird-friendly developments will need to make difficult decisions over which aspects of the bird community to prioritise.

     

Direct and indirect effects of human population density and land use on physical features and invertebrates of Iowa (U.S.A.) streams

To improve understanding of human impacts on headwater stream condition, we quantified relationships between human, terrestrial landscape, and stream system variables in 29 central Iowa watersheds. Across study watersheds, between 0 and 100 % of total land area was characterized as “urban” (developed and barren land), whereas cultivated land constituted between 0 and 71 % of watershed area. Several variables were measured for each stream and associated watershed. Strengths of correlative relationships were used to select variables for path analysis, which we used to gain insight into factors affecting stream condition by evaluating direct and indirect effects of human system variables, terrestrial landscape variables, and physical stream variables on stream invertebrates. Results indicated that in predominantly urban watersheds, contaminant inputs to streams (measured by streamwater conductivity) negatively affected invertebrates, including EPT taxa (Ephemeroptera, Plecoptera, Trichoptera), and streamwater contaminant concentrations increased with impervious surface and human population density in the watershed. In rural watersheds, high streamwater nitrogen concentrations associated with cultivated land were related to declines in invertebrate taxon richness. Independent of land use, invertebrate abundance and taxonomic diversity were positively related to coarse substrate abundance on the streambed. Additionally, stream flow (discharge) increased with watershed area, which in turn increased invertebrate taxonomic diversity. Apparently, mechanisms responsible for human impacts on stream condition in central Iowa depend on dominant land use in the watershed. Additionally, stream ecosystems with high quality benthic habitat, and those located in large watersheds with greater flow, appear to be more resilient to land use effects.     

Stormwater basins of the New Jersey coastal plain: Subsidies or sinks for frogs and toads?

Stormwater basins are commonly employed structures designed to mitigate the negative effects of urban runoff. They are ubiquitous in the urbanized landscape; yet the effect of these basins on anurans has only recently been addressed. Stormwater basins have the potential to influence the breeding distribution of anurans by being sources for some species and sinks for others. Our study aims to determine which species benefit from the existence of stormwater basins, which species are negatively impacted, and what variables are the best predictors of these effects. We monitored thirty-six permanently-ponded basins in southern New Jersey for the presence of anuran larvae and calling males by aural surveys, dip-netting and trapping. We assessed fish presence by interviews, visual encounters, dip-netting, and traps. We divided near shore buffer areas into impervious surface and managed or unmanaged categories for both grass and woody vegetation. Two connectivity metrics, distance to canopied corridor and percent of undeveloped upland were analyzed with ArcGIS. Fish were detected in 92% of the basins. Resistance to fish predation distinguished successful species, those with larvae present, from unsuccessful species, those with calling activity but no larval presence. Connectivity to and availability of terrestrial habitat were significant predictors of the breeding species richness at the basins. Number of calling species increased as access to and amount of terrestrial habitat increased. Therefore, design, management, and placement of permanently-ponded basins can impact anuran communities. Additionally, as wet basins are sources for Rana catesbeiana, where bullfrogs are invasive, basins will likely increase propagule pressure.     

Stream salamander responses to varying degrees of urbanization within Maryland’s piedmont physiographic province

The main objective of this study was to determine how stream salamander assemblages and species respond to varying levels of impervious surface cover within Maryland’s Piedmont physiographic province. We sampled stream salamanders in 21 first-order streams located in watersheds representing a range of impervious surface cover (0–24 %) across the northeastern part of Maryland’s Piedmont region. Habitat data, including stream substrate and riparian characteristics, were measured at each site. Eurycea bislineata was the predominant species of stream salamander encountered during this study (> 99 % of individuals) and most of these individuals were larvae (> 92 %). Consequently, statistical analysis was limited to E. bislineata larvae. We were unable to detect a difference in E. bislineata abundances or body mass index’s among varying levels of impervious surface cover % or any individual site habitat variables alone. These results suggest that in smaller watersheds within the Piedmont of Maryland, local habitat variables, in conjunction with land use variables, are necessary in determining the abundance and body mass index of E. bislineata larvae populations. This study makes a strong case for halting the over-simplification of the relationship between urbanization and the presence/abundance of stream salamanders.

     

Influence of urbanization on the occurrence and activity of aerial insectivorous bats

Activity and species-specific responses of insectivorous bats in different urban-forest conditions provides a general perspective on the adaptability and vulnerability of bat species towards urbanization intensity. Here we evaluated species richness and activity patterns of aerial insectivorous bats across an urbanized landscape in the highlands of Chiapas, in Mexico. Acoustic monitoring of echolocation calls was conducted for 27 nights over a period of four months. Species richness and relative activity of insectivorous bats were estimated in a landscape with different conditions of urbanization intensity: urban areas, non-urban and forest areas. We identified a total of 14 bat species and three phonotypes. Bat species richness and relative activity was similar (X² = 0.568, gl = 2, p > 0.05), but species composition differed among conditions. We observed a significant higher occurrence of Bauerus dubiaquercus, Eptesicus brasiliensis and Myotis californicus in forest sites. Urban sites presented higher occurrence of Molossus rufus and phonotype Molossidae 2, while non-urban sites presented a higher occurrence of Eptesicus furinalis and phonotype Molossidae 2. We were able to identify bat species according to their relative activity in relation todifferent landscape conditions. Species of the Molossidae family presented the highest activity in urban sites, which was positively affected by the number of streetlights, while species of the Vespertilionidae presented the highest activity in forest sites, which was positively related totree density. While urbanization tends to diminish native biodiversity and alter faunal communities, our results show a similar richness and relative activity of aerial insectivorous bats along the urban ecosystem. The effect of urbanization intensity becomes more apparent in species-specific bat activity; the response of species towards particular habitat conditions depends on local habitat quality and characteristics (i.e., presence of streetlights, vegetation cover and tree density).     

Effects of urbanization on macroinvertebrates in tributaries of the St. Johns River, Florida, USA

The effects of urbanization on hydrology, water quality and macroinvertebrates were examined in 7 headwater tributaries of the St. Johns River in the Atlantic Coastal Plain of northeast Florida. All streams had sandy substrata and drained small catchments (24?C231?ha) that ranged from 0 to 51% total impervious area (TIA). Streams unaffected by urbanization had intermittent stream flow and completely dry channels for several weeks in autumn and spring. Urbanized streams always possessed channel water, but 2 streams ceased flowing and became stagnant in autumn and spring. Principle components analysis of chemical and physical measures (i.e. conductivity, nutrients, pH, metals, and stream flow) produced one axis (PCA1) that explained 54% of the total variation among the streams. The variables that loaded negatively on this axis were associated with low flows, while the variables that loaded positively were associated with urban land-use. PCA1 was also positively associated with %TIA. Macroinvertebrate richness ranged from 27 to 45 taxa and was positively associated with %TIA. Macroinvertebrate biomass ranged from 3 to 45?g AFDM/m² and showed a significant, exponential relationship with PCA1 (r ²?=?0.93) with greatest biomass occurring at intermediate %TIA. Invertebrate community structure in the urbanized streams appeared to be mainly influenced by hydrologic factors (perennial vs. intermittent flow regimes). The effects of urbanization on both hydrological and biological variables among the study streams were apparent, but also influenced by site-specific conditions.