大鼠胰岛细胞的分离、纯化和培养研究

目的研究Wistar大鼠胰岛细胞分离、纯化和培养的改良方法和条件,为进行细胞电生理实验提供胞膜完整、活性良好的胰岛细胞。方法水合氯醛腹腔麻醉,采用胶原酶ⅴ逆行灌注、原位消化及Ficoll-400梯度离心的方法分离、纯化大鼠胰岛,并分离、培养胰岛单细胞。结果采用该方法分离、纯化胰岛的获得率稳定;急性分离、培养的胰岛细胞12h～24h贴壁,（4～5）d胰岛细胞生长状态最佳,胞膜完整,折光性好。结论逆行灌注、原位消化及Ficoll-400梯度离心为一种有效的分离、纯化胰岛细胞的方法,培养的胰岛细胞（4～5）d达到最佳状态,适合进一步研究的要求。     

企業培訓促進業務增長——輝瑞的成功企業培訓計劃(英文)

在知識經濟時代,員工的知識和技能正逐漸成為企業最有價值的資產,成為企業競爭優勢的源泉。輝瑞公司的企業培訓是由其企業價值觀決定的,這種企業價值觀促使輝瑞實施著覆蓋全員並因材施教的富有彈性的培訓方案。此外,輝瑞為員工提供了充分透明的職業發展規劃,公司的每一位員工都知曉如何才能被提升以及公司對其提升後的期望,從而使得培訓真正成為了激勵員工的手段。同時,輝瑞的培訓是基於嚴格的培訓業務管理的,而且,輝瑞的培訓對管理者和下屬都有著一套動態的培訓效果的評估與回馈體系。     

Mergers and acquisitions in the light of 1992 — Ensuring strategic good sense underpins corporate ambitions

The prospect of executing acquisitions in Europe currently generates more excitement than almost any other item on the corporate agenda. Venturing out of the home market is regarded once more as a test of management virility.Yet without sound strategic analysis coupled with a careful review of management capacity, ventures abroad are highly risky and may represent poor value for shareholders. This paper returns to the fundamentals required for a methodical review of the merits of expansion by acquisition in unfamiliar territory. It concludes that it is important to marry the theoretical analysis with the most careful commercial review.     

人口城镇化背景下的户籍制度变迁--石家庄市户籍制度改革案例分析

石家庄市的户籍制度改革坚持两个基本条件,即收入来源和住房,放开了7种人的入市条件,大大降低了农民进入大城市的门槛,解决了进城农民的户口问题,推进了城镇化速度,为我国户籍制度改革提供了值得借鉴的宝贵经验.这次改革的消极影响并没有人们想象的那么严重,出现的一些问题也将随着各项配套政策的实行得到解决.     

21世纪中国人口问题透析

江泽民总书记在全国人口、资源、环境座谈会上提出应该重视和研究人口问题 ,面对 2 1世纪经济全球化、信息化等社会和自然科学的发展 ,结合其对人口科学和人口管理带来革命性的影响 ,本文分析了人口总量控制与低生育水平下的生育政策、劳动人口数量的增长与就业等八方面的问题 ,从一种不同的视角来看待 2 1世纪中国人口问题 ,提出了自身独到的见解。     

Effect of frequent WBC treatments on the back pain therapy in elderly men

Abstract

Cryotherapy is the application of a stimulus of a cryotherapeutic temperature below ?100?°C in a period of 1–3?min in order to stimulate and use physiological reactions of human body to cold. It can be applied to specific body parts or to a whole body. Whole-body cryotherapy is a treatment method applied in treatment of motor organ issues, nervous system diseases, psychiatry, dermatology and laryngology. The research group consisted of 80 male in the age range of 65–77 suffering from chronic, lasting more than 3 months, lower back pain. The subjects qualified to the research were divided into two groups. Group A consisted of 40 patients who participated in whole-body cryotherapy (WBC) twice a week. Group B also contained 40 patients who participated in WBC whole week. Examinations were conducted twice. The first one was conducted before the commencement of the treatment while the second one after the therapy was over. The results of the research did not show any statistically significant improvement in patients from Group A. However, the results obtained by Group B have proven significant condition improvement and enable the researchers to conclude that WBC is effective in treating patients with lower back pain.