Single-cell antisense RNA amplification and microarray analysis as a tool for studying neurological degeneration and restoration

Neurodegenerative diseases typically affect subpopulations of neurons. Characterizing these vulnerable cells and identifying the factors that make them susceptible to damage while neighboring cells remain resistant are essential to the understanding of molecular pathogenesis that underlies neurodegenerative diseases. Classically, molecular analysis of the central nervous system involves the identification and isolation of an anatomic region of interest; next, the relevant tissue is pulverized, and the resulting homogenate is analyzed. Although this method provides useful data, its effectiveness diminishes when used in areas of high cellular diversity or in instances in which one cell type is lost as a consequence of selective cell death or quiescence. A technique that affords the ability to assess molecular events in a very precise anatomical site would provide a powerful tool for this research discipline. In this review, we discuss the amplification of messenger RNA from single neural cells and the subsequent use of the RNA to probe DNA microarrays in an effort to create cell-specific molecular profiles. Specifically, recent work in single-cell expression profiling in Alzheimer's and Huntington's diseases is discussed. We also review some new work with neural stem cells and their application to restorative neurobiology. Finally, we discuss the use of cell-specific molecular profiles to better understand the basics of neuronal cell biology.     

Inclusive Governance and “Minority” Groups: The Role of the Third Sector in Wales

Following the establishment of a national legislature in Wales in 1999 the third sector has entered into a pioneering cross-sectoral partnership with the Welsh government. This paper presents the results of a research project that has studied the new structures of devolved governance through the expectations and participation of voluntary organizations representing three marginalized or minority groupings: women, disabled people, and those from an ethnic minority background. The findings reveal that despite varying levels of expectation expressed by minority voluntary groups, active engagement of minority groups in policy making has been a feature of the Assembly's first months. Nevertheless, formidable challenges face both sectoral partners in the new system of governance, not least in creating organizational structures that facilitate partnership working in the devolved polity.     

Homeless Affiliation, Location, and Sender Address in Return of Lost Letters

A field study used 1008 lost letters to test the hypotheses that the differences in returned responses would be attributable to the addressee (i.e., ending homelessness-control or preventing it among youth, veterans, and lesbians) and especially to a socially deviant addressee, that cultural differences between distribution locations might influence returned responses of lost letters, and that sender address (i.e., urban, rural, control) would also influence returned responses. Both addressees' affiliations and distribution location were associated with returned responses, with the lowest rates being for the Prevent Lesbian Homelessness Program affiliate overall and for the city of Pensacola, respectively. Antigay prejudice toward a deviant group may still be strong even when a helping act can be performed without intimate contact involving members of deviant group. Neither distribution location nor the sender or return address conditions were associated with different returned responses. Further testing of the lost letter technique, as a measure of public response, may be warranted.     

Training Mental Health Professionals on Ethical Issues and HIV/AIDS

This report describes the importance of ethics training for mental health professionals working with persons infected or affected by HIV/AIDS. We first describe three major ethical dilemmas (confidentiality, duty to warn, and suicide) faced by mental health providers serving persons with HIV/AIDS, and the legal and clinical aspects of these dilemmas. We present data from the South Texas AIDS Training Project describing the types of mental health professionals who have attended workshops on ethical issues and HIV/AIDS. Finally, we report observations about the impact of the ethics training sessions on the participants' knowledge and attitudes.     

Predictors of Adolescent Adjustment: Parent-Peer Relationships and Parent-Child Conflict

A telephone survey of 114 mothers and their sons, 132 mothers and their daughters, 64 fathers and their sons, and 64 fathers and their daughters investigated the predictors of adolescent adjustment, as separately estimated by parents and their children. Results revealed that the best predictors of adolescent adjustment were the level of conflict within the household over mundane domestic matters and the parent's general disposition towards the child's friends. When results were broken into parent-child gender dyads (mother-son, mother-daughter, father-son, father-daughter), it emerged that the father's perceptions tended to be the dominant predictor of adolescent male adjustment.     

Being Affected: Spinoza and the Psychology of Emotion

This paper describes the relevance of Spinoza's Ethics for contemporary thought on the psychology of emotion. Spinoza's account of the passions completely inverts the Cartesian primacy given to mind. For Spinoza the critical task is to formulate an ethics of knowing, which begins with an understanding that body and mind are two attributes of the same substance. Increasing the capacity of the body to both be affected and affect others is the means by which the knowing subject progresses. The article sketches out the key concepts involved in this system and shows how they sensitize us to a post-cognitive understanding of emotion in Proust's Swann's Way.     

Targeting disadvantage in agriculture

The effectiveness of the main policy instrument targeting support at farmers in disadvantaged areas of the UK from 1976 to 2000 (the Hill Livestock Compensatory Allowance Scheme) is evaluated for Northern Ireland, using recursive linear programming models and input–output analysis. Capital, income and employment effects of the Scheme in its latter years are estimated and assessed against declared policy objectives. It is estimated that average farm cash incomes would have been between 17 and 31 per cent lower in the absence of HLCA payments, while farmer net worth would have fallen by approximately 1 per cent annually. In aggregate, some 1000 jobs were sustained by the Scheme and incomes and profits in the region were boosted by £9 million each year. The main economic effect arising from the introduction of a successor area-based subsidy, will be a redistribution of payments from farms with relatively high stocking rates (predominantly larger units) to those operating more extensively (mostly smaller, part-time farms).     

Sexual Harassment as an Exercise of Power

This article argues that the key to the explanation as to why sexual harassment is a feature of organizational life lies in the issue of power. Yet there has been little attempt to link sexual harassment with theories or explanatory models of power. This article first takes Lukes's (1986) three‐dimensional model as a framework to explore how harassment may be understood as an exercise of power at different levels then shows how radical feminist and post‐structuralist analyses overlap with and are distinct from Lukes's third dimension of power.