应用改进的荧光原位杂交技术(FISH)定量分析单一细胞内miRNA的表达

在过去的十年,我们对miRNA生源和机理的理解得以快速发展;然而,许多关于miRNA表达与细胞功能如何关联的基本问题依然存在。为了能在未来实现对这个层次的理解,可能需要将单细胞水平miRNA的丰度进行定量,从而绘制细胞表型关系。本文介绍一种高灵敏度并且高效的miRNA检测方法——LNA-ELF-FISH,可以在原位的单一细胞中看见单个miRNA分子     

Are football referees really biased and inconsistent?: evidence on the incidence of disciplinary sanction in the English Premier League

Summary.  The paper presents a statistical analysis of patterns in the incidence of disciplinary sanction (yellow and red cards) that were taken against players in the English Premier League over the period 1996–2003. Several questions concerning sources of inconsistency and bias in refereeing standards are examined. Evidence is found to support a time consistency hypothesis, that the average incidence of disciplinary sanction is predominantly stable over time. However, a refereeing consistency hypothesis, that the incidence of disciplinary sanction does not vary between referees, is rejected. The tendency for away teams to incur more disciplinary points than home teams cannot be attributed to the home advantage effect on match results and appears to be due to a refereeing bias favouring the home team.     

Technology and Family Violence in the Context of Post‐Separated Parenting

Whilst the advent of new and increasingly accessible communication technologies undoubtedly provides new, positive, and effective ways for individuals to communicate and connect with their communities, it simultaneously provides additional means and forums for perpetrators to abuse and harass their victims. Furthermore, such technologies enable perpetrators of family violence to overcome geographical boundaries and continue their abuse post‐separation, particularly where there are children of the relationship. This paper reviews and classifies the existing literature on technology‐facilitated abuse, identifying predominant themes in relation to context and focus, as well as the gaps. New forms of criminality as well as new ways to perpetrate existing forms of criminality are described.     

Indirect effects of the Family Check‐Up on youth extracurricular involvement at school‐age through improvements in maternal positive behavior support in early childhood

Extracurricular involvement in the school‐age years has widespread potential benefits for children's subsequent socioemotional development, especially for low‐income youth. However, there is a dearth of research on interventions aimed at increasing school‐age extracurricular involvement in low‐income youth. Thus, the present study aimed to test the collateral effect of a brief, family‐focused intervention for low‐income families, the Family Check‐Up, on children's school‐age extracurricular involvement via improvements in maternal positive behavior support (PBS) in early childhood. The sample (n = 630, 50% female, 50% White, 28% Black/African American) represented a subsample of families from the Early Steps Multisite Study. At the age of two, families were randomly assigned to the Family Check‐Up or Women, Infants, and Children Nutritional Supplement Services as usual. Mother‐child dyads participated in observed interaction tasks at child ages 2 and 3 that were subsequently coded to assess PBS. Primary caregivers reported on children's school‐age extracurricular involvement at ages 7.5, 8.5, and 9.5. Results indicated that although there was not a direct path between intervention status and children's school‐age extracurricular involvement, a significant indirect path emerged from intervention group to changes in PBS between ages 2 to 3 to children's school‐age extracurricular involvement. The results are discussed in terms of implications for designing preventive interventions in early childhood that promote extracurricular involvement at school‐age, particularly for children at risk for maladaptive outcomes.     

Reforming the strategic planning process: Integration of social responsibility and business needs

In this article the author argues that one of the prime organizational imperatives for the development of a responsive, operational, ‘corporate social policy’ must be reform of the strategic planning process. Indeed he argues that this reform is the essential pre-requisite to making the true integration of social responsibility and business needs.