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The great antimicrobial and antioxidant potential of enzymes makes them prone to be used as active packaging materials to preserve food from contamination or degradation. Major drawbacks are connected to the use of enzymes freely dispersed in solution, due to reduced protein stability. The immobilization of enzymes on solid supports to create biocatalytic interfaces has instead been proven to increase their stability and efficiency. In this work, it is shown that enzymes crystallized on hydrogel composite membranes (HCMs) can exert an effective antimicrobial action, thus making the composite membrane and crystals biofilm a potential active substrate for food packaging applications. The antimicrobial hen egg white lysozyme is crystallized on the surface of the hydrogel layer of HCMs, and its activity is determined by measuring the decrease in absorbance of Micrococcus lysodeikticus culture incubated with the specimen. The overall catalytic efficiency of the antimicrobial HCMs increases by a factor of 2 compared to the pure enzyme dissolved in solution at the same quantity. Because the enzyme in crystalline form is present in higher concentration and purity than in the solution, both its overall catalytic efficiency and antimicrobial action increase. Moreover, the hydrogel environment allows a better protein stabilization and retention during crystals dissolution.  相似文献   
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We describe a mixed-effect hurdle model for zero-inflated longitudinal count data, where a baseline variable is included in the model specification. Association between the count data process and the endogenous baseline variable is modeled through a latent structure, assumed to be dependent across equations. We show how model parameters can be estimated in a finite mixture context, allowing for overdispersion, multivariate association and endogeneity of the baseline variable. The model behavior is investigated through a large-scale simulation experiment. An empirical example on health care utilization data is provided.  相似文献   
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Hospital cleaning work, as health care work, is mainly performed by women all over Europe. Hospital cleaning activities represent a poorly studied sector although very important also for patient's health. We applied the Method of Organizational Congruencies to study cleaning work in three typical hospital units (Emergency Room, Haematology, General Medicine) of a roman University hospital where 198 women cleaners work. We analyzed the 731 technical actions performed in the three shifts and the related Organizational Constraints (OC). Working outsourcing, no occupational risks training, washing personal equipment at home, standing, long walking, early morning and night shift in emergency room, high monotony (>10 actions per hour) contact with biological and chemicals materials, risk conditions of accidents, artificial lights, hot microclimate and working in a cure setting represent the main Organizational Constraints. Differences among the three Units are discussed together with the importance of considering cleaning hospital as a preventive action towards hospital clinical risk.  相似文献   
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In the last decades, the use and meaning of the concept of career has profoundly changed, shaped by a ‘new career’ literature rhetoric and a move away from mainstream sociological debate. Our aim in this article is to provide a critical assessment of the concept, and to make a productive contribution to the current debate on careers, and work more generally. Specifically, we seek to: (i) critique the lack of elaboration of the concept within the discipline of sociology in recent years; (ii) reposition the concept of careers as a key sociological category; and (iii) assess and reorient the current meanings of career. After tracing the history of career from linear to boundaryless, we situate the concept in a broader sociological understanding of gender and habitus and structure and agency, and through a methodological discussion of narrative approaches for studying careers. These concepts and approaches are especially effective for understanding careers. Having showed the added value of the concept of career for sociology, we conclude with a research agenda which attempts to overcome the voluntaristic pitfall of its use in recent years and opens up a more thoughtful and articulated understanding of careers for both teaching and research.  相似文献   
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The present short review underlines the role of testosterone (T) in the motivational and satisfaction components of women's sexuality and critically discusses the strategies to treat hypoactive sexual desire disorder (HSDD), a condition of low desire associated with personal and/or interpersonal difficulties, which is more common in surgical menopausal women. There are multiple ways androgens target the brain regions (hypothalamic, limbic and cortical) involved in sexual function and behaviour. Even though circulating available androgens have been implicated in several domains of sexual response, they seem to be related weakly to symptoms, such as low sexual desire, poor sexual arousal, orgasm and diminished well-being in postmenopausal women. The possibilities of treating low sexual desire/HSDD are multifaceted and should include the combination of pharmacological treatments able to maximize biological signals driving the sexual response, and individualized psychosocial therapies in order to overcome personal and relational difficulties. Transdermal T has been shown to be effective at a dose of 300 μg/day both in surgically and naturally menopausal women replaced with estrogen or not, without any relevant side-effects. However, the decision to treat postmenopausal women with HSDD with T is mainly based on clinical judgement, after informed consent regarding the unknown long-term risks.  相似文献   
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Far‐right parties gain in electoral support across the globe. Studies describe this phenomenon either as a cultural backlash or as a reaction to growing economic inequality. The economic inequality perspective suggests that the transforming workforce in post‐industrial societies gives rise to economic insecurity among those who feel left behind. In contrast, the cultural backlash thesis argues that the increasing support for far‐right parties represents a rejection of values such as cosmopolitanism and multiculturalism. More recent scholarship sought to show how economic and cultural factors combined increase the support for the far right. Most of these studies investigate public opinion polls, voting behaviour, and voters´ socio‐economic contexts. This paper reviews these studies and argues that the way in which far‐right political parties construct an interconnection of economic and cultural ideas in discourse is largely neglected in the existing body of literature. The paper concludes that the concept of economic nationalism captures how these two components are intertwined; economic nationalist discourse in far‐right political manifestos and speeches provides a more complete comprehension how public opinion is being shaped. This contribution offers a starting point for future studies to examine how cultural values, such as nationalism, reconstruct and influence articulation of economic policy.  相似文献   
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Abstract

Despite the growing interest in supply chain management (SCM), there is little literature on knowledge and competences needed to properly configure and manage a supply chain. There are some interesting methodologies to map the knowledge and the techniques used in the supply chain of a sector and transfer them in another sector. This is particularly important when cross-fertilisation of practices can support sectors facing global competition as the footwear industry. New business opportunities can in fact be exploited addressing the needs of specific target groups searching for customised fashion and healthy shoes. Based on literature review and case study analysis of twelve companies within the fashion and orthopaedic footwear supply networks, this work compares the relevant processes and the distinctive capabilities necessary in managing supply networks to implement customisation, identifying and highlighting the interchangeable best practices that could serve as a new common knowledge base to be shared by the two sectors.  相似文献   
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ABSTRACT

Recently, distance sampling emerged as an advantageous technique to estimate the abundance of many animal populations, including ungulates. Its basic design involves the random selection of several samplers (transects or points) within the population range, and a Horvitz–Thompson-like estimator is then applied to estimate the population abundance while correcting for animal detectability. Ensuring even coverage probability is essential for subsequent inference on the population size, but it may not be achievable because of limited access to parts of the population range. Moreover, in several environmental conditions, a random selection of samplers may induce very high survey costs because it does not minimize the displacement time of the observer(s) between successive samplers. We thus tested whether two-stage designs – based on the random selection of points and then of nearby samplers – could be more cost-effective, for a given population size and when even area coverage cannot be guaranteed. Here, we further extend our analyses to assess the performance of two-stage designs under varying animal densities.  相似文献   
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