Functional analysis of high-content high-throughput imaging data

High-content automated imaging platforms allow the multiplexing of several targets simultaneously to generate multi-parametric single-cell data sets over extended periods of time. Typically, standard simple measures such as mean value of all cells at every time point are calculated to summarize the temporal process, resulting in loss of time dynamics of the single cells. Multiple experiments are performed but observation time points are not necessarily identical, leading to difficulties when integrating summary measures from different experiments. We used functional data analysis to analyze continuous curve data, where the temporal process of a response variable for each single cell can be described using a smooth curve. This allows analyses to be performed on continuous functions, rather than on original discrete data points. Functional regression models were applied to determine common temporal characteristics of a set of single cell curves and random effects were employed in the models to explain variation between experiments. The aim of the multiplexing approach is to simultaneously analyze the effect of a large number of compounds in comparison to control to discriminate between their mode of action. Functional principal component analysis based on T-statistic curves for pairwise comparison to control was used to study time-dependent compound effects.     

Improved estimation methods for unrelated question randomized response techniques

The randomized response technique (RRT) is an important tool, commonly used to avoid biased answers in survey on sensitive issues by preserving the respondents’ privacy. In this paper, we introduce a data collection method for survey on sensitive issues combining both the unrelated-question RRT and the direct question design. The direct questioning method is utilized to obtain responses to a non sensitive question that is related to the innocuous question from the unrelated-question RRT. These responses serve as additional information that can be used to improve the estimation of the prevalence of the sensitive behavior. Furthermore, we propose two new methods for the estimation of the proportion of respondents possessing the sensitive attribute under a missing data setup. More specifically, we develop the weighted estimator and the weighted conditional likelihood estimator. The performances of our estimators are studied numerically and compared with that of an existing one. Both proposed estimators are more efficient than the Greenberg's estimator. We illustrate our methods using real data from a survey study on illegal use of cable TV service in Taiwan.     

Descriptive Analysis of Foster Care Adoptions in New South Wales,Australia

This paper describes the population of children in care in New South Wales, Australia, who were adopted by their foster families (N?=?372) from 2003 to 2014. Data pertaining to 370 of these adoptees were available and obtained from administrative records accompanying adoption applications. Most of these children had been placed with their subsequent adoptive family during infancy. However, adoption orders occurred much later in their lives. According to the available information at the time of adoption, these children did not suffer the high levels of mental health problems typically identified in populations of children in foster care. Though the data do not include follow-up to determine if the contact agreed upon at the time of adoption orders translated into practice, the most frequently postadoption arrangement recorded was face-to-face contact, four times a year with mother and siblings. The high levels of openness and face-to-face contact in Australian adoptions from care are a significant point of difference with other adoption models, and presents a need for research in the area.

IMPLICATIONS

• Following the change of legislation in New South Wales, Australia, that prioritises open adoption over foster care, social workers in the field of child protection are well placed to assist in this process.

• Social workers can assess children entering care to identify those who will benefit from adoption in a timely manner; undertake casework and administrative aspects of the adoption process; work closely with the child, and birth and adoptive families by delivering pre- and post-adoption supports; and conduct longitudinal research on the outcomes for children adopted from care.

     

Host National Identification of Immigrants in the Netherlands

This study examines immigrants' identification with the host country. We use survey data of more than 1,700 Turkish and Moroccan immigrants and more than 2,200 natives in the Netherlands. We answer four main questions in this study. First, do immigrants have lower national identification than natives? Second, does the level of national identification differ between immigrant groups? Third, do economic and social integration similarly affect national identification among immigrants and natives? And fourth, what are important additional determinants of national identification among immigrants? The results show that, compared to Dutch natives, Turkish but not Moroccan immigrants have lower national identification. Being employed and socially integrated is associated with higher national identification among immigrants as well as natives, but only among immigrants is higher occupational status associated with higher national identification. For immigrants, Dutch language proficiency, perceived discrimination, and contact with natives proved to be important conditions for national identification.     

The importance of being mature: the effect of demographic maturation on global per capita GDP

Given that savings behaviour and worker productivity have strong life-cycle components and given that demographic profiles vary across countries, population age structure should be linked to differences in levels of economic development. In this paper, we measure the economic importance of age structure variation for the global economy. We find that demographic maturation has been associated with nearly half of the evolution of global per capita GDP since 1960. We also find that age structure differences can account for just over half of the variation in worldwide per capita GDP (i.e. the lack of sigma convergence) observed since 1960.

“Post-Gay” Yet? The Relevance of the Lesbian and Gay Scene to Same-Sex Attracted Young People in Contemporary Australia

In recent years, the lesbian and gay “scene” of bars, nightclubs, and dance parties has been undergoing a structural decline in many Western cities. This article aimed to examine the relevance of the scene to same-sex attracted young adults amid these changes. An online survey was conducted with 572 same-sex attracted young women and men in Sydney. Only a minority of respondents participated in the scene at least weekly (16%), and the majority did not regard the scene as important to their leisure time (60%). However, respondents valued the scene as a space to connect with other same-sex attracted people and display same-sex affection without fear of hostility. Lesbian and gay identified respondents tended to be more socially engaged with the scene compared to other same-sex attracted respondents. The findings suggest that while the scene continues to be valued by many same-sex attracted young people, improved social attitudes have made participating in the scene less of an imperative.     

Measuring disparities in access to water based on the normative content of the human right

Measuring access to water in the Sustainable Development Goals era involves taking into account the human rights framework. Therefore, its content should be considered to conceptualize the level of service through adequate indicators and to follow-up inequities reduction at global, national and local level. This research develops and tests a methodology to measure intra-community disparities based on human right to water normative criteria through a stratified sampling, splitting households served by community based organizations and those self-provided. This approach implies considering much reduced populations, thus special care needs to be taken with sample sizes and uncertainty of estimators. The proposed methodology is practical to locate and accurately characterize minority sectors within rural communities and allows moving beyond central-tendency estimators. It implies higher costs for field data collection than traditional approaches, but this can be assumed given the relevance of the approach from a human rights perspective, which calls for adequate tools for equity-oriented policy making at local level. The research point out how results might be used to shape decision-making processes.     

The effects of direct experience on consumer product evaluation

The effects of different sales strategies on consumer product evaluation were investigated. It was expected that direct product experience could decrease economic product valuation. Experiments 1 and 2 studied the effects of product trials and money-back guarantees on consumer willingness to pay (WTP). It appeared that WTP was significantly lower after a product trial than with a money-back guarantee or normal sale. In Experiment 3 a structural equation model showed that product trials had a positive effect on attitude toward the product. Also, a negative effect on WTP was found when the effect was measured directly, but a positive effect when the effect was measured indirectly (through attitude).     

Soil Nutrient Depletion and Population Growth in Sub-Saharan Africa: A Malthusian Nexus?

Soil nutrient mining and other forms of soil degradation threaten future soil productivity, especially in sub-Saharan Africa (SSA). Data from 36 countries in SSA show a significant relationship at the supra-national level between soil nutrient depletion (including erosion), reduced fallow periods, and population pressure and thus illustrate the unsustainable population-agriculture-environment nexus on the continent which so far has been described in case studies only. It appears that Malthusian mechanisms are at work, despite encouraging indications of farmers' concerns and adaptation to changing conditions. It is agreed that a re-examination of the common land degradation assessments and farmers' response to changing conditions would be useful, however, this should not delay other recommended interventions.