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71.
High-content automated imaging platforms allow the multiplexing of several targets simultaneously to generate multi-parametric single-cell data sets over extended periods of time. Typically, standard simple measures such as mean value of all cells at every time point are calculated to summarize the temporal process, resulting in loss of time dynamics of the single cells. Multiple experiments are performed but observation time points are not necessarily identical, leading to difficulties when integrating summary measures from different experiments. We used functional data analysis to analyze continuous curve data, where the temporal process of a response variable for each single cell can be described using a smooth curve. This allows analyses to be performed on continuous functions, rather than on original discrete data points. Functional regression models were applied to determine common temporal characteristics of a set of single cell curves and random effects were employed in the models to explain variation between experiments. The aim of the multiplexing approach is to simultaneously analyze the effect of a large number of compounds in comparison to control to discriminate between their mode of action. Functional principal component analysis based on T-statistic curves for pairwise comparison to control was used to study time-dependent compound effects. 相似文献
72.
Health research traditionally has focused on the health risks and deficits of lesbian, gay, bisexual, transgender, and queer (LGBTQ) populations, obscuring the determinants that can promote health across the life course. Recognizing, appropriately measuring, and rendering visible these determinants of health is paramount to informing appropriate and engaging health policies, services, and systems for LGBTQ populations. The overarching purpose of this article is to provide an overview of the findings of a scoping review aimed at exploring strengths-based health promotion approaches to understanding and measuring LGBTQ health. Specifically, this scoping review examined peer-reviewed, published academic literature to determine (a) existing methodological frameworks for studying LGBTQ health from a strengths-based health promotion approach, and (b) suggestions for future methodological approaches for studying LGBTQ health from a strengths-based health promotion approach. The findings of this scoping review will be used to inform the development of a study aimed at assessing the health of and improving pathways to health services among LGBTQ populations in Nova Scotia, Canada. 相似文献
73.
Marian van Bakel Jan Pieter van Oudenhoven Marinel Gerritsen 《Human Resource Development International》2017,20(3):215-235
Social capital is a crucial factor for expatriates to employ as they cope with the demands of an international assignment. This longitudinal study used a mixed method approach to examine the social support benefits of expatriate contact with a local host. Western expatriates in the Netherlands were randomly divided into two groups: an experimental group (n = 33), that had contact with a Dutch host during 9 months, and a control group (n = 32) with no host. Qualitative methods such as interviews and diaries were included to shed light on the various types of social support that occurred. Results show that local hosts offered all four types of social support: social companionship, informational support, emotional support, and instrumental support. Furthermore, expatriates with a host increased their social capital; they received significantly more social support from host nationals than did those without a host. This study shows that HRD professionals may develop the social capital of expatriates by bringing them into contact with a local host, which can produce more social support from host nationals. Increased social capital may lead to a higher performance at both the individual and organisational levels. 相似文献
74.
Jacqueline A. Miller Edward A. Smith Donna Coffman Catherine Mathews Lisa Wegner 《Journal of research on adolescence》2016,26(4):673-686
Nearly 20% of South African youth experience forced or coerced sexual intercourse. Understanding the factors associated with forced sex is important for informing prevention programs aimed at reducing sexual violence and HIV and AIDS. Multilevel regression models test the association between sexual situation self‐efficacy and forced sex among 2,893 South African adolescents. Findings suggest that youth are more likely to experience forced sex after periods of time when their levels of self‐efficacy are lower than their average levels of self‐efficacy. Furthermore, youth who are lower on their self‐efficacy compared to their peers are more likely to experience forced sex. Implications for prevention research are discussed. 相似文献
75.
Jacqueline Martin Tracy Gladstone Anne Diehl William Beardslee 《Journal of Technology in Human Services》2016,34(3):267-284
This study evaluated the acceptability, feasibility, and satisfaction associated with a newly developed online clinician training program for the Family Talk preventive intervention, both alone and together with a redesigned, shortened, face-to-face component. Fifty-eight predominately in-home therapy clinicians participated in the study. Results indicated that clinician participants found the online training to be enjoyable and comprehensive, and they reported that the most beneficial training package involved the combination of web-based and in-person training. This combined training could efficiently cover necessary didactic material online while also delivering important clinical skill practice and in-person discussion. Exceptions, limitations, and important future research questions are discussed. 相似文献
76.
Prenatal Reflective Functioning and Accumulated Risk as Predictors of Maternal Interactive Behavior During Free Play,the Still‐Face Paradigm,and Two Teaching Tasks 下载免费PDF全文
Hanneke J. A. Smaling Stephan C. J. Huijbregts Jill Suurland Kristiaan B. van der Heijden Judi Mesman Stephanie H. M. van Goozen Hanna Swaab 《Infancy》2016,21(6):766-784
This study examined whether prenatal reflective functioning (RF) was related to mothers’ interactive style across contexts with their 6‐month‐old infants (M age = 6.02 months, SD = 0.41, 54% boys), and to what extent quality of prenatal RF could account for the influence of accumulated risk on maternal interactive behavior. Accumulated risk was defined as the sum‐score of a selection of risk factors that have been associated with suboptimal infant development. Mother–infant dyads (N = 133) were observed during free play, two teaching tasks, and the Still‐Face Paradigm (SFP). Better prenatal RF was associated with more positive maternal behavior in all settings and less negative behavior during teaching and SFP reengagement. Accumulated risk and prenatal RF predicted shared variance in maternal interactive behavior (with unique predictive effects observed only for RF on sensitivity during teaching and SFP play, and for accumulated risk on sensitivity and positive engagement during SFP play, and internalizing‐helplessness during SFP reengagement). Accumulated risk had an indirect effect on maternal sensitivity during teaching and SFP play through prenatal RF. These findings suggest not only that RF may be targeted prenatally to improve mother–infant interactions, but also that enhancing RF skills may ameliorate some of the negative consequences from more stable perinatal risk factors that influence parent–child interactions. 相似文献
77.
The authors investigated intergenerational support exchanges in relation to young adults' life course status. In a sample of 2,022 young adults (ages 18–34 years) in The Netherlands, single young adults reported receiving more advice from parents than married young adults, and those with children of their own received more practical support. Married young adults and young adults with children provided less support to parents than, respectively, single young adults and young adults without children. Congruent with the life course perspective, the authors' findings suggest that the intergenerational support network is both durable and flexible, responding to the resources and needs associated with an individual's life course status. 相似文献
78.
79.
We investigate the implications of balanced consistency and balanced cost reduction in the context of sequencing problems. Balanced consistency requires that the effect on the payoff from the departure of one agent to another agent should be equal between any two agents.
On the other hand, balanced cost reduction requires that if one agent leaves a problem, then the total payoffs of the remaining agents should be affected by the amount
previously assigned to the leaving agent. We show that the minimal transfer rule is the only rule satisfying efficiency and Pareto indifference together with either one of our two main axioms, balanced consistency and balanced cost reduction. 相似文献
80.
Over the past 20 years, rural areas in Western societies have transformed from a production to a consumption space. Much research on rural diversification and revitalization has focused on farmers and their wives. However, it is useful to examine side activities run by non-farm women which have slowly emerged in the last few years. In view of discussions about rural decline, the value of such activities should be looked at in a new light. Although these activities may not significantly reverse unemployment figures, they have the potential to provide the social and emotional ‘glue’ to motivate a household to remain in declining areas as small-scale economic activities contribute to a better quality of life and a higher level of well-being. In this article we draw on the personal stories of women who undertake side activities in the Veenkoloniën, the Netherlands. We explore the factors that enable these women to start a side activity and the related organizational and emotional struggles they face while running such activities within the perimeter of their home. We further highlight the importance of side activities for the empowerment of women in rural households. 相似文献