代谢综合征基础与临床系列研究——GRGDSP/7E3对动脉粥样硬化内皮细胞MMPs的mRNA表达的影响

目的了解GRGDSP/7E3对基质金属蛋白酶（MMP-2,MMP-9）mRNA表达的影响。方法体外培养人脐静脉内皮细胞,分别用酶谱法和逆转录聚合酶链式反应（RT-PCR）检测MMP-2,MMP-9活性和mRNA表达。待细胞融合成单层后,分别加入无血清培养基、静息期血小板、活化后血小板及interleukin-1β（IL-1β）,共同培养60min后,洗去血小板,内皮细胞继续培养6h。上清液及细胞分别用于酶谱法检测MMP-2和MMP-9活性,以及RT-PCR分析mRNA表达。结果内皮细胞与活化血小板共同培养后,酶谱法显示：与对照组相比,未活化的血小板轻微诱导MMP-2和MMP-9分泌,而活化状态的血小板能显著诱导这二者的分泌,与IL-1β的作用相当;而且还可以见到62kDaMMP-2活化形式。加入阻断剂GRGDSP以及GPⅡb/Ⅲa单克隆抗体（7E3）作用后检测发现,MMP-2和MMP-9分泌减少。与活化血小板共同培养后的内皮细胞表面表达uPAR和MT1-MMP以及上清液中MMP-2的mRNA与对照组相比明显增高,而静息状态的血小板作用不显著。加入GRGDSP或7E3后,mR-NA的表达降低。结论①活化状态的血小板与IL-1β的作用相当,能显著诱导MMP-2和MMP-9分泌;能明显增高内皮细胞（EC）-uPAR和MT1-MMP及上清液中MMP-2mRNA的表达;②GPⅡb/Ⅲa阻断剂可能抑制血小板在不稳定斑块部位聚集、粘附等一系列炎症反应。     

武汉市关山地区“城中村”改造问题调查报告

一、调查背景与目的 随着改革开放的深入,城市经济得以迅速发展,城市的大规模建设,也使城市面貌得以迅速改观.     

东北方言中的万能动词——整

方言是极具地方和民族性格特征的一种表现,也是特殊的文化现象。辽宁是一个多民族聚居地区,东西南北各有不同的语音语调。用文字的形式表述方言的特点,也是本刊的一个尝试。意于相声、小品之外,更多了解辽宁人的不同生活侧面。本刊今起将开设"东北话"栏目。相信它能通过对我们习以为常的一些用词用语加以归纳梳理,帮助国内外读者走进辽宁人丰富多彩的真实生活之中。     

Adult Drug Treatment Courts: A Review

Drug treatment courts (DTCs), an alternative to traditional criminal courts, provide an innovative way to legally process some drug offenders. The origin and recent growth of the drug court system in the USA can be explained as an unintended consequence of a failing 'war on drugs'. In this article, we discuss the spread of adult DTCs throughout the USA, the main components of drug courts, controversies surrounding DTCs, and some criticisms of drug courts. We summarize the recent evaluations of adult DTCs, and highlight various DTC factors that have been found to reduce individual drug use and criminal activity. We also offer suggestions for future research on DTCs and conclude with policy recommendations.     

POTENTIAL COMPETITION AND POSSIBLE COLLUSION IN FOREST SERVICE TIMBER AUCTIONS

Potential competition significantly affects the size of winning bids in Forest Service sealed-bid timber auctions and has little effect on winning bids in oral auctions. Winning sealed bids depend even more, however, on actual competition, a result suggesting collusion. This explanation is supported using an index representing the likelihood an auction was rigged. Preclusive bidding (a type of collusion) in oral auctions is indicated by a positive relationship between hauling distances and the size of winning sealed bids. Comparisons of winning-bid variances, overbids, and numbers of bidders across auction type support this explanation of oral auction prices.     

Matrix-trace Cauchy-Schwarz inequalities and applications in canonical correlation analysis

Various matrix-trace Cauchy-Schwarz and related inequalities involving positive semidefinite matrices are obtained. Applications of some of these results to canonical correlation analysis are presented.     

清末“急务”与“本源”的失调——以鸦片禁政期间的财政窘况为背景

清末新政时期的编练新军和振兴实业分别被谕旨称为“急务”和“本源”,这是新政时期国家振兴的重要国策。两项国策的推行均需要有大量的财政投入。由于推行鸦片禁政,国产和进口鸦片税厘大幅度缩减,影响了中央和地方财政的拓展。在清廷的督催下,练兵新政不断得到强化,成为中央和地方财政支出的大项,这必然导致振兴实业因缺少足够的资金注入和宽松的税收环境而举步维艰。尤其是在制定国家预算的过程中,海陆军经费占据了国家财政预算相当高的比例,振兴实业这一国策在财政预算中并未明显地体现出来,形成了练兵“急务”突出,而实业“本源”却不断弱化的现实,两者在财政困绌条件下事实上处于“失衡”状态。朝野各方由此对军政失调、过分突出军备的倾向颇有微词,尤以各种报章的批评言论最为尖锐,国外人士对清廷推行新政中本末倒置现象的批评性观点也通过各种途径体现出来。清亡的原因也可借此得到一定的解释。     

间歇开放系统的可靠性分析与数值计算

研究了一种新型的可靠性系统，即间歇开放系统。在建立数学模型的基础上，我们详细分析了系统有关的可靠性问题，不仅获得时刻ｔ系统失效的概率和（０，ｔ］中系统失效的平均次数的瞬态解，而且也获得其平稳结果。最后用数值计算比较了间歇开放系统和经典系统的差别。