花粉管通道转导外源野生稻DNA进入栽培稻的方法研究

本文报道了在实验室(空调培养)、温室及田间等不同条件下,利用花粉管通道转导外源野生稻 DNA 进入栽培稻,并将 D_0代外源 DNA 重组体幼胚进行培养,威功地获得了一批外源野生稻 DNA 重组体植株的实验方法。结果表明,用花粉管通道转导外源 DNA 的整体系统是简单易行的方法。花粉管通道转导外源野生稻DNA 与 D_0代重组体幼胚离体培养相结合的技术是在分子水平上改良稻种资源和进行水稻分子育种的有效途径。     

Risk Assessment for Aflatoxin: I. Metabolism of Aflatoxin B1 by Different Species

A comparison of the generation and detoxification of reactive aflatoxin B1 metabolites in different species may elucidate why animal species vary widely in sensitivity to aflatoxin B1 carcinogenicity, in addition to offering some perspective on how sensitive man may be to the carcinogenic effects of this mycotoxin. Scientific literature comparing the ability of cellular fractions from different species to metabolize aflatoxin B1 is reviewed. However, in vitro studies exclude components for multiple metabolic pathways, eliminating the possibility of competition between metabolic activation and detoxification. Quantification of metabolic activation by different species from these studies is therefore limited. The species-specific carcinogenic potency of aflatoxin B1 may be reflected in levels of aflatoxin B1-DNA adducts generated in vivo. The current paucity of quantitative information on human DNA adduct levels does not permit comparisons between different species on this basis.     

组件技术和Windows DNA体系结构

主要阐述组件技术和W indows DNA技术,并分析了采用三层结构进行开发的必要性。     

反义寡核苷酸技术

本文基于反义寡核苷酸技术的基本原理，评述了该技术的主要内容和近些年来的研究进展；着重介绍了其在农业、医药和生物学基础研究等方面的应用．此外，还分析了此技术在应用方面存在的一些问题，并展示了其应用前景．     

是达到了最高学术水平,还是罕见的假冒伪劣--评荆贵生主编的《古代汉语》

河南师范大学教授荆贵生宣称由他主编的<古代汉语>达到了最高学术水平,本文则以大量无可辩驳的事实揭露该书是一部罕见的假冒伪劣之作.此外,作者谴责了庸俗的书评,并对如何制止践踏学术道德、违背学术规范的不良现象,提出了自己的看法.     

“大范围抽血采验DNA”现象研究

随着DNA技术在刑事司法实践中的应用,"大范围抽血采验DNA"这种侦查方式被侦查机关广泛使用,其对刑事案件的侦破起到了一定积极作用,但也存在较大缺陷。根据对"大范围抽血采验DNA"的产生发展及其实际应用状况等方面的分析,这一侦查方式不仅侵害公民合法权益,而且成本高昂、成功机率不高。应规范侦查机关的侦查权,完善刑事诉讼法。     

Horn Clause Computation with DNA Molecules

In this paper, we will propose Horn clause computation as an underlying computational framework of DNA computer. Horn clause program is a subclass of the formulas of first order logic and has close relation to PROLOG language. The computational power of Horn clauses was discussed in (Tärnland, 1977) and it was shown that a finite set of Horn clauses is computationally equivalent to Turing Machine. Furthermore, it should be noted that Horn programs are adequate for representing nondeterministic computation. Thus, its parallel implementation with huge number of molecules might have possibility to overcome the computational power of conventional computers. It should also be noted that the clear logical semantics of Horn programs might enable us to accept it as a higher-level programming language of DNA computer. The aim of this paper is to propose an experimental method for implementing deduction with a subclass of Horn programs, called simple Horn programs. The computational power of this model is theoretically investigated and demonstrated with some applications to NP-complete problems.     

Estimation of parameters in DNA mixture analysis

In [7], a Bayesian network for analysis of mixed traces of DNA was presented using gamma distributions for modelling peak sizes in the electropherogram. It was demonstrated that the analysis was sensitive to the choice of a variance factor and hence this should be adapted to any new trace analysed. In this paper, we discuss how the variance parameter can be estimated by maximum likelihood to achieve this. The unknown proportions of DNA from each contributor can similarly be estimated by maximum likelihood jointly with the variance parameter. Furthermore, we discuss how to incorporate prior knowledge about the parameters in a Bayesian analysis. The proposed estimation methods are illustrated through a few examples of applications for calculating evidential value in casework and for mixture deconvolution.     

DNA指纹与性状间的相关度量

根据资料总体分布特征，推导出ＤＮＡ 指纹与两种类型性状间的相关度量公式。论述了使用该公式筛选标记性状指纹的合理性，同时给出了相应的计算和统计分析方法