代谢综合征基础与临床系列研究——GRGDSP/7E3对动脉粥样硬化内皮细胞MMPs的mRNA表达的影响

目的了解GRGDSP/7E3对基质金属蛋白酶（MMP-2,MMP-9）mRNA表达的影响。方法体外培养人脐静脉内皮细胞,分别用酶谱法和逆转录聚合酶链式反应（RT-PCR）检测MMP-2,MMP-9活性和mRNA表达。待细胞融合成单层后,分别加入无血清培养基、静息期血小板、活化后血小板及interleukin-1β（IL-1β）,共同培养60min后,洗去血小板,内皮细胞继续培养6h。上清液及细胞分别用于酶谱法检测MMP-2和MMP-9活性,以及RT-PCR分析mRNA表达。结果内皮细胞与活化血小板共同培养后,酶谱法显示：与对照组相比,未活化的血小板轻微诱导MMP-2和MMP-9分泌,而活化状态的血小板能显著诱导这二者的分泌,与IL-1β的作用相当;而且还可以见到62kDaMMP-2活化形式。加入阻断剂GRGDSP以及GPⅡb/Ⅲa单克隆抗体（7E3）作用后检测发现,MMP-2和MMP-9分泌减少。与活化血小板共同培养后的内皮细胞表面表达uPAR和MT1-MMP以及上清液中MMP-2的mRNA与对照组相比明显增高,而静息状态的血小板作用不显著。加入GRGDSP或7E3后,mR-NA的表达降低。结论①活化状态的血小板与IL-1β的作用相当,能显著诱导MMP-2和MMP-9分泌;能明显增高内皮细胞（EC）-uPAR和MT1-MMP及上清液中MMP-2mRNA的表达;②GPⅡb/Ⅲa阻断剂可能抑制血小板在不稳定斑块部位聚集、粘附等一系列炎症反应。     

基于SVM的综合实力评估系统研究

简略介绍了支持向量机的基本思想 ,研究其在综合评价中的应用。分析了对大学进行实力评估的过程 ,建立基于SVM的评价系统。比较新评价系统和采用其他方法如PCA (PrincipalComponentAnalysis主元分析 ) ,Fisher等建立的评价系统所分别取得的拟合效果。结果表明 :采用支持向量机设计的评价系统思路清晰 ,操作简单并且能取得更为理想的评估结果。     

Applicability of a Neuroprobabilistic Integral Risk Index for the Environmental Management of Polluted Areas: A Case Study

Recently, we developed a GIS-Integrated Integral Risk Index (IRI) to assess human health risks in areas with presence of environmental pollutants. Contaminants were previously ranked by applying a self-organizing map (SOM) to their characteristics of persistence, bioaccumulation, and toxicity in order to obtain the Hazard Index (HI). In the present study, the original IRI was substantially improved by allowing the entrance of probabilistic data. A neuroprobabilistic HI was developed by combining SOM and Monte Carlo analysis. In general terms, the deterministic and probabilistic HIs followed a similar pattern: polychlorinated biphenyls (PCBs) and light polycyclic aromatic hydrocarbons (PAHs) were the pollutants showing the highest and lowest values of HI, respectively. However, the bioaccumulation value of heavy metals notably increased after considering a probability density function to explain the bioaccumulation factor. To check its applicability, a case study was investigated. The probabilistic integral risk was calculated in the chemical/petrochemical industrial area of Tarragona (Catalonia, Spain), where an environmental program has been carried out since 2002. The risk change between 2002 and 2005 was evaluated on the basis of probabilistic data of the levels of various pollutants in soils. The results indicated that the risk of the chemicals under study did not follow a homogeneous tendency. However, the current levels of pollution do not mean a relevant source of health risks for the local population. Moreover, the neuroprobabilistic HI seems to be an adequate tool to be taken into account in risk assessment processes.     

二世策墨林诺们罕活佛圆寂日期与地点续考

二世策墨林诺们罕是清代西藏著名活佛，原籍甘肃省洮州厅卓尼土司辖区，曾任西藏摄政（1819-1844）、第七十三任甘丹赤巴和十世达赖喇嘛的正师傅，是西藏历史上权势最大、结局最为悲惨的摄政活佛。道光二十四年（1844年）六月其被革职查办，拘禁拿问，次年（1845年）奉旨流放黑龙江，给披甲人为奴。国内外史学界对其最终结局、圆寂时间、圆寂地点、享年岁数及其呼毕勒罕三世策墨林活佛的出生年份历来众说纷纭。中国第一历史档案馆藏军机处录副奏折表明：成丰十年四月十九日（1860年6月8日），二世策墨林诺们罕活佛在新疆喀喇沙尔乌讷恩苏珠克图旧土尔扈特蒙古南部落的札萨克卓哩克图汗旗圆寂，享年69岁（1792-1860年），遗骨奉旨安葬于该旗境内的佑安寺（又称永安寺、巴伦台黄庙），其呼毕勒罕三世策墨林活佛出生于成丰十一年（1861年），而非其他年份。     

Risk,trust and knowledge networks in farmers’ learning

This study reports on New Zealand dairy farmers’ access to and use of information as mediated through conditions of risk and trust within the context of their interpersonal social networks. We located participants’ reports of their information use within their perceived environments of trust and risk, following Giddens's [1990. The consequences of modernity. Polity Press, Stanford, CA] typology of trust and risk in pre-modernity and modernity. The research participants were constant users of interpersonal and print information from numerous sources, and monitored their incoming data in the light of strategic needs, reflecting their roles as both farming practitioners and business owners. Socio-spatial knowledge networks (SSKNs) combine individuals’ explanatory cognitive models of information acquisition and use with a micro-geographical analysis of their interpersonal networks. The participants showed characteristics of pre-modern, modern and even post-modern society in respect of their use of complex interactional forms, as well as a blending of individualistic and communitarian practices and concerns in their professional and personal lives.     

矩阵方程XA＝B的亚（半）正定自共轭四元数矩阵解

给出了四元数体Ｑ上ｎ×ｎ分块矩阵为亚（半）正定自共轭矩阵的一个充要条件，进而给出了Ｑ上矩阵方程ＸＡｎｍ＝Ｂｎｍ有亚（半）正定自共轭四元数矩阵解的充要条件及解集合的显式表示，从而推广改进了数城上线性方程组的反问题及矩阵反问题的相应结果．     

土工地锚加固软土地基的工作机理研究

根据土工地锚的构成形式，分析了土工地锚这一新技术在薄层软土地基加固中的机理和作用，并提出了相应的分析方法．研究表明土工地锚在软基加固中具有广阔的应用前景．     

基于平衡计分卡的政府传统行政模式的转型

以传统政府模式转型成“企业化政府”模式为典型,把已经在企业内部取得瞩目成就的平衡计分卡管理模式引入“企业化政府”管理模式,增强政府改革的可行性和有效性。     

骨干网IPv4/IPv6协议转换网关设计

阐述了骨干网上部署IPv6网络中,解决既要满足IPv4和IPv6网络互联互通的需要,同时减少IPv4地址消耗的问题。比较了几种比较成熟的IPv4/IPv6转换技术,提出了在IPv4/IPv6网络边界的协议转换网关上使用改进的NAPT-PT协议转换方案,指出了在城域网环境下采用NAPT-PT方案的不足和实现过程中的解决办法。     

基于虚拟仪器的土壤水分测试系统开发

采用PCl-812PG数据采集卡,基于虚拟仪器技术,以Delphi作为平台开发了土壤含水量测试系统,对AZS -2土壤水分传感器的功能进行了扩展。标定结果及实际应用表明,此系统性能稳定,精度较高。