文明基因的解读——水流动力学的启示

通过比较河流动力学与海洋动力学的不同,揭示水流动力学机制对世界文明的深远影响。海洋动力学机制使西方文明彰显出开放、多元的梯形结构,河流动力学机制则构造了大河文明的基因。     

基因脉冲导入仪及其在动物肿瘤治疗上的应用

本文介绍了电穿孔技术的原理和天津理工大学自行研制的基因脉冲导入仪 LN-301的系统组成。使用该系统在昆明小白鼠进行肿瘤治疗实验。实验结果表明：基因脉冲导入仪有利于肿瘤的治疗。     

利用DNA来建构古埃及18王朝的王室家谱

把生物学的技术应用在考古学上 ,就形成了一门新兴的分支学科———分子考古学 ,它可以对许多用传统的考古方法没法解决或者是结论不太确凿的问题作出新的解释 ,比如 :有关人类的起源问题、动物的驯养和植物的栽培等。本文介绍的是用DNA手段来研究古埃及 18王朝的王室家谱 ,得出了一些与传统观点不一致的假设。     

美国藏学家金•史密斯收集藏文文献的“西去东归”

金?史密斯（Ellis Gene Smith）先生由美国摩门教著名的家族成员转归藏文文献的收集、整理与研究,成为国际知名藏学家及藏传佛教文献资料中心（TBRC）的创始人,为当代世界藏学研究与发展做出了重要的贡献。金?史密斯先生收集藏文文献具有种类齐全、数字化传承、开放利用、坚持不懈等特点,充分体现了其深远的智慧和高尚的人格品德。2007年10月26日,金?史密斯先生决定将其毕生收集的藏文文献捐赠给西南民族大学,而且还为这些被捐赠的藏文文献支付了从美国纽约到中国成都的所有运输费用。西南民族大学秉承金?史密斯先生深远的智慧和周全的考虑,将其所捐赠的藏文文献珍藏在新建的“金?史密斯藏学文献馆”中供藏学学习者和研究者查阅与研究,实现了金?史密斯先生希望所捐赠的藏文文献在中国乃至世界发挥最大利用价值的宿愿。     

Global Markov models for eukaryote nucleotide data

We discuss the quite remarkable global Markovian structure of the nucleotides in eukaryotic DNA strands with special emphasis on (i) the similarity property for intra- species chromosomes and (ii) the reversibility property for the two (complementary) strands of a chromosome.     

基因脉冲导入仪在电化学疗法中的应用

本文介绍了电化学疗法的原理和天津理工大学自行研制的基因脉冲导入仪LN-301的系统组成。在此基础上,利用基因脉冲导入仪LN-301及配套的针电极在小鼠身上进行了电化学疗法实验。通过电化学疗法对接种有S180的小鼠进行对比实验。实验结果表明:应用抗癌药物环磷酰胺(CTX)和博莱霉素(BLM)的ECT组的抑瘤率分别为60.9%和70.4%,与传统的治疗方法相比抑瘤率显著提高。     

Network-based genomic discovery: application and comparison of Markov random field models

Summary.  As biological knowledge accumulates rapidly, gene networks encoding genomewide gene–gene interactions have been constructed. As an improvement over the standard mixture model that tests all the genes identically and independently distributed a priori , Wei and co-workers have proposed modelling a gene network as a discrete or Gaussian Markov random field (MRF) in a mixture model to analyse genomic data. However, how these methods compare in practical applications is not well understood and this is the aim here. We also propose two novel constraints in prior specifications for the Gaussian MRF model and a fully Bayesian approach to the discrete MRF model. We assess the accuracy of estimating the false discovery rate by posterior probabilities in the context of MRF models. Applications to a chromatin immuno-precipitation–chip data set and simulated data show that the modified Gaussian MRF models have superior performance compared with other models, and both MRF-based mixture models, with reasonable robustness to misspecified gene networks, outperform the standard mixture model.     

Partial least squares Cox regression for genome-wide data

Most methods for survival prediction from high-dimensional genomic data combine the Cox proportional hazards model with some technique of dimension reduction, such as partial least squares regression (PLS). Applying PLS to the Cox model is not entirely straightforward, and multiple approaches have been proposed. The method of Park et al. (Bioinformatics 18(Suppl. 1):S120–S127, 2002) uses a reformulation of the Cox likelihood to a Poisson type likelihood, thereby enabling estimation by iteratively reweighted partial least squares for generalized linear models. We propose a modification of the method of park et al. (2002) such that estimates of the baseline hazard and the gene effects are obtained in separate steps. The resulting method has several advantages over the method of park et al. (2002) and other existing Cox PLS approaches, as it allows for estimation of survival probabilities for new patients, enables a less memory-demanding estimation procedure, and allows for incorporation of lower-dimensional non-genomic variables like disease grade and tumor thickness. We also propose to combine our Cox PLS method with an initial gene selection step in which genes are ordered by their Cox score and only the highest-ranking k% of the genes are retained, obtaining a so-called supervised partial least squares regression method. In simulations, both the unsupervised and the supervised version outperform other Cox PLS methods.     

聚合酶链反应(PCR)技术与基因扩增分析仪器(PCR仪)

较为全面地论述了聚合酶链反应(PCR)技术与基因扩增分析仪器(PCR仪)。较为详细地阐述了PCR基本原理、PCR结果的检测与分析、实时定量PCR技术、影响PCR效果的因素、PCR技术的应用、PCR仪器的现状及其发展方向。