Functional analysis of high-content high-throughput imaging data

High-content automated imaging platforms allow the multiplexing of several targets simultaneously to generate multi-parametric single-cell data sets over extended periods of time. Typically, standard simple measures such as mean value of all cells at every time point are calculated to summarize the temporal process, resulting in loss of time dynamics of the single cells. Multiple experiments are performed but observation time points are not necessarily identical, leading to difficulties when integrating summary measures from different experiments. We used functional data analysis to analyze continuous curve data, where the temporal process of a response variable for each single cell can be described using a smooth curve. This allows analyses to be performed on continuous functions, rather than on original discrete data points. Functional regression models were applied to determine common temporal characteristics of a set of single cell curves and random effects were employed in the models to explain variation between experiments. The aim of the multiplexing approach is to simultaneously analyze the effect of a large number of compounds in comparison to control to discriminate between their mode of action. Functional principal component analysis based on T-statistic curves for pairwise comparison to control was used to study time-dependent compound effects.     

“I am a fat baby,who moved to a fat child,who moved to a fat teenager,who moved to a fat adult”: Women’s reflections of a lifetime of body and weight concern

The present study explored how women aged 50–65 years reflect and make meaning of a lifetime of body and weight struggles. Seven purposefully selected women with longstanding body image challenges participated in interviews and reflected on their perceptions, thoughts, and emotions around their body and weight since childhood. Findings revealed consistent and consuming concerns about the body; prevalent body-related self-conscious emotions; influential social experiences that impact physical self-perceptions; and enduring impacts of weight bias, stigma, and discrimination. Collectively, these findings provide support for the stability of body disturbances and highlight the need to explore the unique body-related narratives of women in midlife.     

不忘初心：基于央视宣传片百年政党多模态形象建构研究

央视形象宣传片《我是谁》是中国共产党建党95周年以来的首部公益广告,具有重要的时代意义和研究价值.结合视觉设计语法、评价理论和“宣传片”的图像、文本和声音语料建立“宣传片”多模态形象诠释框架,探索“宣传片”建构党的形象的过程与范式.研究发现:一是“宣传片”图像的再现意义体现接地气的创意理念,互动意义展现与受众间的平等权势关系,构图意义揭示青年群体是目标受众的精准定位;二是“宣传片”文本重点建构人际意义的协商语义,从客观视角而不是主观情感刻画党员群体爱岗敬业的务实作风和百年政党一心为民的赤子之心;三是“宣传片”音乐、旁白等声音能够有效增强受众全方位了解党的形象塑造,从而尽快接受党为人民服务的不变“初心”.     

后殖民主义视角下的热播美剧及对策研究

文章应用爱德华·萨伊德的后殖民主义理论作为武器,选取美剧中的中国元素作为研究对象,首先解析了美剧中作为他者消费性的、建立在西方种族优越构筑机制之下的中国元素的显著特点;然后分析了这一现象可能导致的社会问题及解决这些问题的相应对策。     

Body image,aging, and identity in women over 50: The Gender and Body Image (GABI) study

We conducted a qualitative study of 1,849 women over age 50 to capture the thoughts, feelings, and attitudes that women at middle age have about their bodies and the experience of aging. Via an open-ended question online survey, four primary themes emerged: (a) the physical and psychological experience of aging; (b) the injustices, inequities, and challenges of aging; (c) the importance of self-care; and (d) a plea for recognition of the need to maintain a contributory role in society. Results highlight the complexities of women’s psychological and physical aspects of aging and point toward important topics worthy of further study in this growing population.     

Using institutional ethnography's ideological circle to portray how textually mediated disability discourse paralysed a Supreme Court of Canada ruling

Persons with disabilities created through texts as damaged goods are being ‘cured’ by workplace accommodation, a textually mediated work process. Prior to 1999, workplace accommodation in the Canadian federal public service signified the adjustments necessary to enable a disabled worker to fit into a workplace designed for able-bodied workers. In 1999, a Supreme Court of Canada (SCC) ruling known as Meiorin turned this accommodation provision on its head. Instead of being focused on individual-level fixes so that a disabled worker could be accommodated in an existing workplace, this legislation required employers to transform their workplaces so as to make them ready to receive as many different types of workers as reasonably possible from the outset. Using Dorothy E. Smith's institutional ethnography, I use my experiences as a disability rights activist employed in the Canadian federal public service to explicate how textually mediated disability discourse paralysed this groundbreaking SCC ruling.     

抗战时期中国形象的建构：基于国际友人视角

抗战时期,美国作家温台尔·威尔基在所撰写的《天下一家》一书中,通过自己在中国的现场体验和对中国政治、经济及民众抗战的切身感受,在美国人民面前呈现出新的中国形象。这种新形象的建立,为美国对华援助起到了极大的推动作用。     

高校图书馆女性馆员外在形象之我见

高校图书馆主要的职责是给读者提供服务,服务过程中馆员的形象直接或间接影响者服务质量。高校图书馆女性馆员占60%以上,女性馆员的形象在一定程度而言代表着高校图书馆的形象,文章从女性馆员的仪表风度、谈吐举止、着装搭配等等几方面来探讨高校图书馆女性馆员的外在形象。     

Research initiation based on idea-circles: from research object to co-actor

This article details an evaluation of a research project based on participatory research methods organized by the Swedish Disability Federation from 2008 to 2011. In Sweden there has been a lack of productive dialogue with the traditional academic world and the question was raised whether proposals for future research would be different if disabled people formulated them. Nine idea-circles with disabled participants and invited researchers from fields of interest close to the participants produced ideas, developed out of the life experience of being a disabled person. These ideas were developed into 72 research drafts that often reflected key advocacy areas, rather than operationalized research issues. The adjustment from the familiar discourse of political struggle to discussion of research was a complicated process for many. When asked to prioritize among areas for research, the representatives from the disability movement chose areas that are not stressed in mainstream disability research in Sweden.     

新型城镇化中的老城形象优化策略

城镇更新是城镇发展中"修复振兴、激发活力"的动力机制。而在拓展城镇规模,建设形成新城镇形象的过程中,保护老城风貌并采取各种优化、更新要素的方法传承形象特色,是不同历史阶段化解形象危机和传承文化特色的有效途径。尤其是在当前城乡一体、资源统筹的新型城镇化语境下,探索优化老城形象的策略,对未来城、镇、村的层级、梯度发展、增强城镇活力具有重要意义。本文从滁州老城物质风貌的显性与隐性形象要素入手,对形象要素"分裂、重构、整合"出的"生态、建筑、文化"等形态风貌关系进行分类分析,提出优化策略,为保护老城形象、传承文脉、转型发展注入新的活力。