食用菌细胞融合新品种金凤2-1的生物学特性研究

金凤 2— 1是利用金针菇和凤尾菇的细胞原生质体 ,采用细胞融合技术选育出来的兼有亲本优良特性的新品种[1] 。研究了温度、湿度、pH值、光线、培养基质、栽培料等因素与金凤 2— 1菌丝生长和子实体生长的关系。结果表明 :金凤 2— 1母种最适宜在N源丰富的松针培养基、蛋白胨培养基、酵母膏和天然加富培养基上生长 ;菌丝生长温度范围为 5～ 35℃ ,最适温度为 2 0～ 2 5℃ ,菌丝生长pH值范围是 3～ 10 ,最适pH为 5～ 6 ;栽培料以杂木屑和杂木屑与棉籽壳混合料最好。该菌株菌丝生长速度快 ,生活周期短、转潮快、抗逆性强 ,所采三潮菇生物转化率为 113.8%。     

Wistar大鼠小肠系膜淋巴管的胚胎发育

应用酶组织化学、透射电子显微镜及淋巴管墨汁活体注射法对大鼠小肠系膜淋巴管的发育进行了研究 .结果表明 :( 1 )小肠系膜淋巴管的雏形出现于胚胎 1 6天 ;( 2 )小肠系膜淋巴管弓有 1— 3级 ,分布不规则 ;( 3)胚胎期与成年期小肠系膜淋巴管内皮的超微结构有差异 .     

相对论速调管中调制腔的三维设计

根据二维模拟中电子束在调制腔中所需要的微波场,采用三维电磁场模拟软件进行调制腔的三维设计。在相对论速调管放大器中输入微波的馈入调制腔内,其作用场为TM01模;由于调制腔需要从外部馈入微波,通常为一种较复杂的三维结构。通过三维带电子束的电磁场模拟,验证了三维设计调制腔在给定输入功率条件下对电子束的调制效率及其三维设计。     

异构小蜂窝网络移动性管理研究综述

新一代移动通信网络不断演进为高密集、无定型、立体分层的异构网络,移动性管理旨在保证用户设备（user equipment,UE）移动通信服务的连续性和无缝切换及降低信令开销等。移动性管理问题正成为异构小蜂窝网络（heterogeneous and small cell networks,HetNets）中无线资源管理需要应对的关键性挑战之一。阐述了移动性管理的一般性问题,根据移动性管理的主要特征给出了一个综合分类：整体切换性能增强、小蜂窝发现与识别增强和无线链路失效（radio link failure,RLF）恢复增强。此外,依据此分类对近年来第三代合作伙伴计划（3rd genera-tion partnership project,3GPP）成员在无线接入网（radio access network,RAN）标准化工作组历次会议中提出的主要移动性管理增强方案进行了分类阐述和性能对比。对异构小蜂窝网络的移动性管理所面临的挑战和未来研究的趋势进行展望,并在此基础上进行了总结。     

小檗碱透皮制剂对小鼠骨髓细胞的微核效应

本文采取小鼠骨髓细胞微核试验方法,研究小檗碱透皮制剂对体细胞的诱变性。结果表明：阴性对照组微核率为1．125±0．9％,环磷酰胺阳性对照组的微核率为5．125±0.976％,与阴性对照组比较,差异极显著（P＜0．01）;小檗碱透皮制剂低、中、高剂量组微核率分别为1．125±0．69%、1．5±0．9%、1.875±0．787‰,与阴性对照比较,差异均不显著（P＞0．06）,试验结果为阴性。小檗碱透皮制剂对小鼠骨髓细胞不具诱变性。     

用直线法计算TEMcell中高次模截止频率

提出用直线法计算ＴＥＭｃｅｌｌ中高次模截止频率，求出了多个ＴＥ和ＴＭ模的截止频率值。所得结果与其他方法的结果吻合，表明直线法的精度高、计算量小，是很有效的方法。     

新技术背景下骨髓捐献补偿机制的法学思考——以美国弗林案为视角

在弗林案中．美国法院认为通过外周血干细胞单采技术从血中获取的造血干细胞是血液的子部分而非骨髓的子部分,因而《国家器官移植法》的补偿禁令不适用于此项新技术。尽管此判决具有些许瑕疵,但其确立了骨髓捐献的补偿机制,对增加骨髓造血干细胞移植具有积极的政策意义。对于采用外周血干细胞单采技术进行的骨髓捐献,我国应允许补偿机制,现阶段应优先考虑社会规律而非自然规律,因而对此问题宜准用《献血法》而非《人体器官移植条例》。     

骨髓基质干细胞复合PLGA支架修复兔关节软骨缺损

目的探讨骨髓基质干细胞复合PLGA支架对兔关节软骨损伤的修复作用。方法以聚乳酸/聚羟基乙酸共聚物、磷酸三钙制备支架。取新西兰乳兔骨髓分离BMSCs,传代后接种于支架。54只新西兰大白兔,建立膝关节软骨缺损模型,随机分为模型对照组、支架组和支架+BMSCs组。结果光镜示BMSCs在支架内生长良好;膝关节活动度和移植组织的平均厚度与周边正常软骨平均厚度百分比显著高于模型对照组(P<0·01)。结论 PLGA支架复合BMSCs提高了兔关节软骨缺损的修复效果。     

光电池非线性区PN结光生伏特效应的研究

根据光电池线性区应用叠加定理建立的PN结光生伏特效应理论，阐述了PN结光生伏特效应在光电池非线性区的应用。     

Risk Assessment of Listeria monocytogenes: Impact of Individual Cell Variability on the Exposure Assessment Step

Recently, the lag phase research in predictive microbiology is focusing more on the individual cell variability, especially for pathogenic microorganisms that typically occur in very low contamination levels, like Listeria monocytogenes. In this study, the effect of this individual cell lag phase variability was introduced in an exposure assessment study for L. monocytogenes in a liver paté. A basic framework was designed to estimate the contamination level of paté at the time of consumption, taking into account the frequency of contamination and the initial contamination levels of paté at retail. Growth was calculated on paté units of 150 g, comparing an individual-based approach with a classical population-based approach. The two different protocols were compared using simulations. If only the individual cell lag variability was taken into account, important differences were observed in cell density at the time of consumption between the individual-based approach and the classical approach, especially at low inoculum levels, resulting in high variability when using the individual-based approach. Although, when all variable factors were taken into account, no significant differences were observed between the different approaches, allowing the conclusion that the individual cell lag phase variability was overruled by the global variability of the exposure assessment framework. Even in more extreme conditions like a low inoculum level or a low water activity, no differences were created in cell density at the time of consumption between the individual-based approach and the classical approach. This means that the individual cell lag phase variability of L. monocytogenes has important consequences when studying specific growth cases, especially when the applied inoculum levels are low, but when performing more general exposure assessment studies, the variability between the individual cell lag phases is too limited to have a major impact on the total exposure assessment.