应用琼扩试验确定鸡IBD首免日龄的研究

应用琼脂免疫扩散试验监测雏鸡IBD母源抗体阳性率下降规律,以及不同日龄免疫后IBD抗体阳性率变化情况,筛选出雏鸡IBD的合理免疫程序。本试验将雏鸡分成8个组隔离饲养,分别在5、8、10、14、18、22、26日龄用B_(87)中等毒力疫苗免疫,首免后10d用同一疫苗二免。结果10、14日龄首免组。在二免后10d AGP阳性率达60％以上,其AGP阳性率上升快,免疫空白期短,明显优于其他日龄免疫组。     

高炉废渣制备白炭黑工艺探索

本文分析了小高炉炼铁废渣的主要成份，通过一系列的实验，对酸的浓度，酸的用量，反应温度，ｐＨ值等工艺条件优选，得到一种最佳制备白炭黑的工艺条件。     

猪水肿毒素琼脂扩散沉淀反应方法的建立

采用滤膜除菌、生物大分子浓缩胶浓缩、戊二醛灭活的方法制备的EHEc水肿毒素抗原,可与其兔高免血清和鸡高免血清发生沉淀反应。对5株EHEc进行了分析,并与小白鼠毒性试验结果进行了比较,证明该沉淀反应具有高度的特异性。     

Optimal lamination test of ethylene vinyl acetate sheets for solar modules

ABSTRACT

Solar power is inexhaustible and has become one of the most appreciated alternative energy choices. In the development stage, solar modules are subjected to relevant reliability tests to ensure a long lifetime and optimal power generation efficiency. After the lamination process, the performance of solar modules is closely related to the degree of crosslinking of ethylene vinyl acetate (EVA) sheets. Traditionally, the degree of crosslinking on EVA sheets is obtained using the chemical extraction method to measure the gel content of these sheets. Motivated by lamination data, this study first constructed a statistical model to describe the relationship between the degree of crosslinking on EVA sheets and lamination time. Next, under the specification limits of the gel content of EVA sheets, the optimal lamination time of solar modules was derived, and the optimal allocation for measuring EVA sheets was addressed. The chemical extraction method is time consuming and leads to high pollution. The latest method is differential scanning calorimetric (DSC), which measures the curing degree of EVA sheets as the degree of crosslinking on these sheets. This study determined the specification limits of the curing degree under the DSC method. An example is presented to elucidate the proposed procedure.     

Effects of testosterone treatment on body composition in males with testosterone deficiency syndrome

Abstract

Objective: We evaluated the safety of testosterone treatment and its efficacy on body composition in males with testosterone deficiency syndrome (TDS) over 24 months.

Methods: 50 males aged 50–65 years with TDS (Aging Males Symptoms Scale [AMS]?>?26 and calculated free testosterone [cFT] 250?pmol/l) were administered 50?mg testosterone gel daily for one year. During the second year, patients received 1000?mg of testosterone undecanoate every 2–3 months. Outcome measures were clinical chemistry values and total testosterone; sex hormone-binding globulin and cFT, changes in AMS and International Prostate Symptom Score; and changes in body composition measured by dual-energy-x-ray absorptiometry.

Results: There were no clinically significant changes in clinical chemistry safety parameters. There were significant improvements in both total and cFT and in AMS scores after three months (p?<?0.001). Lean mass increased 2.35% at 12 months and 4.5% at 24 months, but proportionally more muscle mass was gained in arms and legs than in the trunk. Fat mass decreased 4.2% at 12 months and 9.1% at 24 months.

Conclusions: Testosterone treatment in males with TDS leads to body changes affecting lean and fat mass with significant improvement in AMS scores, and has an excellent safety profile.     

产卵期东方蝾螈(Cynops orientalis)同工酶的分析研究

采用聚丙烯酰胺垂直平板电泳方法，研究了东方蝾螈七种组织（小肠、肝、肾、脑、心脏、性腺和尾肌）中的LDH同工酶和EST同工酶，电泳结果显示，不同个体不同组织的LDH同工酶均有二条酶带（LDH1和LDH5），数量上组织特异性不明显，但在显色深度上存在组织差异，其中性腺的LDH1带显色最浅，这表明组织细胞中同时合成了A和B亚单位，但并非随机组合为五种四聚体，显示出经典的五条带．EST同工酶谱可分为四个区共19条带，具有明显的组织特异性，雌雄个体同组织间的差异也很显著。     

小鹅瘟病毒的增殖与沉淀抗原的制备

取小鹅瘟病毒经尿囊腔接种12日龄非免疫鸭胚进行传代增殖，收集尿囊液后采用聚乙二醇沉淀法制备小鹅瘟病毒沉淀抗原，经琼脂扩散试验检测，结果表明：该抗原能够与小鹅瘟阳性血清出现沉淀线，而且与小鹅瘟标准抗原的沉淀线相吻合，与其他常见的禽病血清不发生交叉沉淀反应．本次制备的小鹅瘟沉淀抗原可以用于免疫效果的监测和流行病学调查。     

交联聚合物动态成胶及运移的室内模拟研究

室内采用长岩芯填砂装置模拟了交联聚合物注入、成胶封堵和运移过程。室内模拟结果表明,注入的调剖体系能够达到设计的封堵位置,在注入过程中体系受稀释、剪切和吸附等因素影响,其浓度和成胶前的初始黏度沿程呈下降趋势。在实验所用的浓度（0.2%HPAM）条件下,孔隙介质中的堵剂体系形成有效堵塞的时间显著延长,是静态条件下的6 倍;从不同部位堵剂取样成胶后黏度和岩芯封堵前后注水压力变化情况看,有效封堵距离r 小于设计封堵距离r′,0.67r′ < r < r′。在后续水驱作用下,一部分凝胶能够沿高渗层发生运移,产生深部调驱作用。     

一种基于用溶胶凝胶法固定HRP的化学发光型H_2O_2传感器

报道了一种基于用溶胶凝胶法固定HRP的化学发光型H2 O2 传感器 ,此传感器用于流动注射化学发光分析体系 ,可实现对H2 O2 的在线检测 ,测定的线性范围为 10～ 2 0 0 0 μmol/L ,检测限为 1 2 μmol/L ,10次测定的RSD为 2 1%。