血沉、白细胞计数和C反应蛋白在糖尿病足评估中的作用

目的本文旨在评估炎性因子而对不同程度的糖尿病足病患者作出早期判断的策略。方法对68例不同程度的糖尿病足病患者入院分别进行血沉、白细胞计数和C反应蛋白的测定并作比较。结果 C反应蛋白在2级~4级的糖尿病足病患者中是最有价值的标记物,而血沉、白细胞计数无法预示其程度。结论在不同程度的糖尿病足病患者中C反应蛋白是一个有价值的预测因子。     

CeO2／蛇纹石体系对饮用水的脱氟行为研究

本文探讨了利用均相沉淀法制备的二氧化铈的脱氟行为,并利用蛇纹石作为载体,对CeO2/蛇纹石体系的除氟行为及最佳条件进行了进一步的研究,结果表明：均相沉淀法得到的二氧化铈除氟效率高,除氟容量可达11.76mg/g。以二氧化铈;蛇纹石为1：10的混合体系的除氟容量大大高于其他方法处理过的蛇纹石体系,其附近氟容量达到0.568mg/g。     

Extended pairwise local alignment of wild card DNA/RNA sequences using dynamic programming

Optimal string alignment is used to discover evolutionary relationships or mutations in DNA/RNA or protein sequences. Errors, missing parts or uncertainty in such a sequence can be covered with wild cards, so-called wild bases. This makes an alignment possible even when the data are corrupted or incomplete. The extended pairwise local alignment of wild card DNA/RNA sequences requires additional calculations in the dynamic programming algorithm and necessitates a subsequent best- and worst-case analysis for the wild card positions. In this paper, we propose an algorithm which solves the problem of input data wild cards, offers a highly flexible set of parameters and displays a detailed alignment output and a compact representation of the mutated positions of the alignment. An implementation of the algorithm can be obtained at https://github.com/sysbio-bioinf/swat+ and http://sysbio.uni-ulm.de/?Software:Swat+.     

Accelerating inference for diffusions observed with measurement error and large sample sizes using approximate Bayesian computation

In recent years, dynamical modelling has been provided with a range of breakthrough methods to perform exact Bayesian inference. However, it is often computationally unfeasible to apply exact statistical methodologies in the context of large data sets and complex models. This paper considers a nonlinear stochastic differential equation model observed with correlated measurement errors and an application to protein folding modelling. An approximate Bayesian computation (ABC)-MCMC algorithm is suggested to allow inference for model parameters within reasonable time constraints. The ABC algorithm uses simulations of ‘subsamples’ from the assumed data-generating model as well as a so-called ‘early-rejection’ strategy to speed up computations in the ABC-MCMC sampler. Using a considerate amount of subsamples does not seem to degrade the quality of the inferential results for the considered applications. A simulation study is conducted to compare our strategy with exact Bayesian inference, the latter resulting two orders of magnitude slower than ABC-MCMC for the considered set-up. Finally, the ABC algorithm is applied to a large size protein data. The suggested methodology is fairly general and not limited to the exemplified model and data.     

Soy protein based supplementation supports metabolic effects of resistance training in previously untrained middle aged males

Abstract

Objective.?To determine changes in body composition, physical performance, metabolic and hormonal parameters induced by lifestyle counselling, resistance training and resistance training with soy protein based supplemention in middle aged males.

Design.?Randomised controlled study consisting of resistance training without (RT-G) or with (RTS-G) a soy protein based supplement and a control group with lifestyle education only (LE-G).

Subjects.?Forty healthy middle aged men (50–65 years, BMI 25–29.9 kg/m²).

Measurements.?Changes in body weight (BW) and waist circumference (WC) were measured and body composition (BC), fat mass (FM), lean body mass (LBM) were measured by skin fold anthropometry at baseline and after 12 weeks of intervention. In addition, changes in physical fitness, metabolic and hormonal parameters (lipids, glucose, fructosamines, insulin, insulin-like growth factor-1, Leptin, human growth hormone, dehydroepiandrosterone, testosterone, hs-CRP, Il-6) were evaluated.

Results.?Thirty-five participants completed the 12 week study. No significant changes in BW were noted although RM and WC dropped and LBM increased after training, particularly in the RTS group (FM 22.6?±?5.5?kg to 21.2?±?4.7?kg; LBM 68.5?±?7.2?kg to 70.1?±?7.4; p?<?0.01). Subjects in the RTS group experienced more pronounced improvements in the strength measurements than the RT group. After the training intervention there were significant changes in hormonal and metabolic parameters as well as in glycemic control, particularly in the RTS group.

Conclusions.?Our data suggest that resistance training, particularly in combination with a soy protein based supplement improves body composition and metabolic function in middle aged untrained and moderately overweight males.     

长江经济带工业环境效率差异及其影响因素——基于超效率DEA Malmquist Tobit模型

以长江经济带11个省市为研究对象,运用超效率DEA模型对2005—2014年各省市的工业环境效率进行静态评价。实证结果表明： 2005—2014年长江经济带整体工业环境效率水平较高,但各地区之间仍然存在一定差距,其中浙江、江苏等地环境效率值较高,云南、贵州等地较低;11个省市的全要素环境效率的增长率平均值大于1,技术进步是工业环境效率变动的主要因素;产业结构和环境管制对工业环境效率产生积极影响,外商投资和人口密度对工业环境效率产生消极影响。     

27类蛋白质折叠子的识别及其位点的统计分析

从蛋白质的氨基酸序列出发,采用全体分类的分类策略识别27类折叠子.当使用5种矩阵打分的方法识别27类折叠子时,预测结果并不理想,进一步使用了以矩阵打分方法得到的分值及氨基酸的二肽组分共同作为参数的离散增量算法,取得了较好预测效果.总的预测成功率达到了45.43%.     

价格支持政策对大豆全要素生产率的影响机制分析

在超效率DEA模型测算价格支持政策时期大豆TFP的基础上,使用DID模型分析价格支持政策对大豆TFP的净效应及其影响路径。结果表明：价格支持政策的实施抑制了大豆TFP的有效提升,但存在结构性差异,市场化改革有助于改善大豆TFP;价格支持政策对大豆TFP产生的抑制作用持续性并不强;价格支持政策形成“消极生产率效应”的作用路径是其未带来有效的收益激励,财富水平和市场扭曲抑制了豆农改善生产要素配置状况的积极性。考虑到短期内无法缩小同玉米单产差距的实际,大豆生产者补贴政策若不能给予农户稳定的收益预期,则对改善TFP的作用不应过高估计。为此,在大豆生产者补贴政策调整和修正的过程中应加强系统的战略设计,由单一化的补贴激励转向多元化的政策支持。     

HIV-1gp41NHR序列靶点专一性的荧光共振能转移测定

目的确定并利用荧光共振能转移法（FRET）对以HIV-lgp41N端七联重复序列（NHR）为靶点的融合抑制剂进行筛选和作用机制研究。方法FRET采用金属络合物多肽技术设计针对不同结合位点、结合强度可调、涵盖全部NHR序列的靶点和探针,对HIV融合抑制剂进行高通量筛选。由于HIV在进行膜融合时其gp41的N端NHR和C端CHR可形成稳定的六螺旋结构,因此,利用圆二色谱仪对FRET所使用的靶点／探针对的结合强度进行验证,确定对应的靶点／探针对可形成稳定的六螺旋结构;同时,借助细胞活性测试测定抑制荆的活性,验证FRET是否可用于筛选以HIV-1gp41NHR为靶点的抑制剂。结果与结论FRET中使用的靶点／探针均可形成螺旋度较高的六螺旋结构,其中Fe（Env2．0）,／CP2及Fe（Env5．0）,／CP5形成的01．螺旋度分别高达89．6％和84．7％。FRET所使用的靶点／探针对专一性强、结合作用强,可用于进行HIV一1融合抑制剂的筛选和机制研究。     

在蛋白质分子中激发的由孤立子引起的红移(英文)

在蛋白质分子中若考虑amide-I振子与分子链的谐振动而引起的孤立子将引起激子能量的降低，于是在这些材料的红外吸收谱中将引起峰值的红移。若从文中的理论出发，采用格林函数方法，可求出所形成孤立子的束缚能为14．4～19．65cm－1，与从红外吸收实验中所测得的红移化15cm－1基本符合。结果表明在有机蛋白质分子中确实存在这类孤立子激发，也表明文中的理论是可信的。