非言语交际与外语教学

非言语交际作为言语交际的重要组成部分,在外语教学中,起着不可忽视的作用。恰当地使用非言语交际行为,能够创建良好的师生关系,提高学生学习兴趣。本文分析了非言语交际行为的重要性及其在外语课堂教学中的表现形式和作用。     

Stepchildren’s Communicative and Emotional Journey from Divorce to Remarriage: Predictors of Stepfamily Satisfaction

This study explores the emotional and communicative profiles of young adults who have experienced a parental divorce and the emergence of a stepfamily to better understand influences on stepfamily satisfaction. Results of regression analyses indicate that strong negative emotions experienced at the time of the divorce are a negative predictor of current stepfamily satisfaction, even after controlling for age at the time of the divorce. In addition, level of open self-disclosure to a stepparent, although not parents, is a positive predictor of current stepfamily satisfaction. Finally, the extent to which the father has now fully explained the circumstances of the divorce is a positive predictor of current stepfamily satisfaction, whereas the extent to which mother has now fully explained the circumstances of the divorce is a negative predictor of current stepfamily satisfaction. These findings are discussed and future directions for research on stepfamily interaction and satisfaction are offered.     

Temporal prediction of future state occupation in a multistate model from high-dimensional baseline covariates via pseudo-value regression

In many complex diseases such as cancer, a patient undergoes various disease stages before reaching a terminal state (say disease free or death). This fits a multistate model framework where a prognosis may be equivalent to predicting the state occupation at a future time t. With the advent of high-throughput genomic and proteomic assays, a clinician may intent to use such high-dimensional covariates in making better prediction of state occupation. In this article, we offer a practical solution to this problem by combining a useful technique, called pseudo-value (PV) regression, with a latent factor or a penalized regression method such as the partial least squares (PLS) or the least absolute shrinkage and selection operator (LASSO), or their variants. We explore the predictive performances of these combinations in various high-dimensional settings via extensive simulation studies. Overall, this strategy works fairly well provided the models are tuned properly. Overall, the PLS turns out to be slightly better than LASSO in most settings investigated by us, for the purpose of temporal prediction of future state occupation. We illustrate the utility of these PV-based high-dimensional regression methods using a lung cancer data set where we use the patients’ baseline gene expression values.     

Sometimes Words Just Ain't Enough – Enhancing the Contribution of Children in Therapy through Creative Expression

The meaningful inclusion of children in family therapy is an ideal that therapists readily align with and yet struggle to implement effectively. Within a practice that relies heavily on verbal expression, young children provide a unique challenge for therapists, who require developmentally appropriate opportunities for the child to express, document, and share his or her experience in all its richness and colour. In this paper the author utilises art therapy to support greater inclusion of children in family therapy by capitalising on their creative talents and facilitating the child's visual story within the family dialogue. This can assist in the formulation and treatment of systemic issues.     

日语复合动词中的甚词研究

日语复合动词里的部分后项动词以及部分前项动词在构词过程中产生了语法化变化,失去了该词汇所具有的本来意义。其语法作用相当于汉语中表示极端程度的甚词表达,主要用来表示程度或者强调等意义。但是,日语甚词表达和汉语甚词表达之间在所用词汇上没有交集。所以,不能使用汉语甚词表达去套用日语的甚词表达。     

构建我国基因专利池的设想

专利池是专利的"强强联合",是技术垄断者的再次集中。在发达国家纷纷发展基因专利技术,并试图对我国进行技术包围之际,我们应该未雨绸缪,思考本国基因专利池的构建。本文从基因技术的发展现状和专利制度本身出发,分析了专利池的优点和专利池的组建经验。同时对我国的基因产业实力进行合理的评估,提出了建立我国基因专利池的4种可能,论述了应该如何构建基因专利池以及基因专利池的反垄断规制。以期加速生物时代我国基因专利池的构建,并促进我国基因技术的研究和应用,推动基因产业的发展。     

舞蹈与文学的"通"与"异"

舞蹈与文学有许多相通之处,如创作目的、表现手法、艺术形象等,都是人们自我表达的媒体;而不同之处在于艺术表现形式上,舞蹈是肢体语言,文学是文字语言.如果将两者有机地联系起来,并加以相互应用,将对舞蹈艺术教育起到良好的促进作用.     

烟草硫氧还蛋白基因NtTRX-hl的克隆和功能分析

从烟草中克隆了硫氧还蛋白基因NtTRX-hl并推导出其氨基酸序列,系统进化关系分析表明,NtTRX-hl与小麦、大麦、拟南芥等多种植物的TRX蛋白有很高的同源性.构建高效表达载体,诱导表达重组菌得到分子大小为22.26 kD的融合蛋白,与推测的分子量一致.用胰岛素和二硫苏糖醇作底物进行酶活性测定表明,Nt-TRX-hl具有活性.采用生物信息学的方法和工具分析了NtTRX-hl蛋白的生化参数和亚细胞定位,预测了该蛋白的高级结构.     

番茄遗传转化体系的建立

为了获得带有白细胞介素2基因的转基因番茄植株,本研究运用农杆菌介导法,探究了影响遗传转化的因素,建立了较完善的番茄遗传转化体系。结果表明最优化的转化条件为:Km的筛选浓度为30mg/L,头孢曲松钠的抑菌浓度为500mg/L,侵染时间采用1~2min,并且抑菌7d后加入筛选压。得到的抗性植株经PCR检测为阳性,初步证明外源基因il-2已经整合到番茄基因组中。     

bFGF发酵工艺参数正交优化试验

通过正交实验研究了bFGF发酵的各主要影响因素对于最终产物的数量和纯度的关系,从而确定发酵的最适工艺条件。结果表明当温度控制在37℃,pH值7.0,发酵时间10小时,IPTG诱导浓度为100μg/mL,消泡剂为5 mL/L时,调节溶解氧在40-60%之间对于整个发酵过程是非常有利的。