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251.
High-content automated imaging platforms allow the multiplexing of several targets simultaneously to generate multi-parametric single-cell data sets over extended periods of time. Typically, standard simple measures such as mean value of all cells at every time point are calculated to summarize the temporal process, resulting in loss of time dynamics of the single cells. Multiple experiments are performed but observation time points are not necessarily identical, leading to difficulties when integrating summary measures from different experiments. We used functional data analysis to analyze continuous curve data, where the temporal process of a response variable for each single cell can be described using a smooth curve. This allows analyses to be performed on continuous functions, rather than on original discrete data points. Functional regression models were applied to determine common temporal characteristics of a set of single cell curves and random effects were employed in the models to explain variation between experiments. The aim of the multiplexing approach is to simultaneously analyze the effect of a large number of compounds in comparison to control to discriminate between their mode of action. Functional principal component analysis based on T-statistic curves for pairwise comparison to control was used to study time-dependent compound effects. 相似文献
252.
The varying coefficient (VC) model introduced by Hastie and Tibshirani [26] is arguably one of the most remarkable recent developments in nonparametric regression theory. The VC model is an extension of the ordinary regression model where the coefficients are allowed to vary as smooth functions of an effect modifier possibly different from the regressors. The VC model reduces the modelling bias with its unique structure while also avoiding the ‘curse of dimensionality’ problem. While the VC model has been applied widely in a variety of disciplines, its application in economics has been minimal. The central goal of this paper is to apply VC modelling to the estimation of a hedonic house price function using data from Hong Kong, one of the world's most buoyant real estate markets. We demonstrate the advantages of the VC approach over traditional parametric and semi-parametric regressions in the face of a large number of regressors. We further combine VC modelling with quantile regression to examine the heterogeneity of the marginal effects of attributes across the distribution of housing prices. 相似文献
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Local influence is a well-known method for identifying the influential observations in a dataset and commonly needed in a statistical analysis. In this paper, we study the local influence on the parameters of interest in the seemingly unrelated regression model with ridge estimation, when there exists collinearity among the explanatory variables. We examine two types of perturbation schemes to identify influential observations: the perturbation of variance and the perturbation of individual explanatory variables. Finally, the efficacy of our proposed method is illustrated by analyzing [13] productivity dataset. 相似文献
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M. Moghimbeygi 《Journal of applied statistics》2017,44(7):1282-1295
One of the important topics in morphometry that received high attention recently is the longitudinal analysis of shape variation. According to Kendall's definition of shape, the shape of object appertains on non-Euclidean space, making the longitudinal study of configuration somehow difficult. However, to simplify this task, triangulation of the objects and then constructing a non-parametric regression-type model on the unit sphere is pursued in this paper. The prediction of the configurations in some time instances is done using both properties of triangulation and the size of great baselines. Moreover, minimizing a Euclidean risk function is proposed to select feasible weights in constructing smoother functions in a non-parametric smoothing manner. These will provide some proper shape growth models to analysis objects varying in time. The proposed models are applied to analysis of two real-life data sets. 相似文献
257.
Estimation and Inference Procedures for Semiparametric Distribution Models with Varying Linear‐Index
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More flexible semiparametric linear‐index regression models are proposed to describe the conditional distribution. Such a model formulation captures varying effects of covariates over the support of a response variable in distribution, offers an alternative perspective on dimension reduction and covers a lot of widely used parametric and semiparameteric regression models. A feasible pseudo likelihood approach, accompanied with a simple and easily implemented algorithm, is further developed for the mixed case with both varying and invariant coefficients. By justifying some theoretical properties on Banach spaces, the uniform consistency and asymptotic Gaussian process of the proposed estimator are also established in this article. In addition, under the monotonicity of distribution in linear‐index, we develop an alternative approach based on maximizing a varying accuracy measure. By virtue of the asymptotic recursion relation for the estimators, some of the achievements in this direction include showing the convergence of the iterative computation procedure and establishing the large sample properties of the resulting estimator. It is noticeable that our theoretical framework is very helpful in constructing confidence bands for the parameters of interest and tests for the hypotheses of various qualitative structures in distribution. Generally, the developed estimation and inference procedures perform quite satisfactorily in the conducted simulations and are demonstrated to be useful in reanalysing data from the Boston house price study and the World Values Survey. 相似文献
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We address the issue of model selection in beta regressions with varying dispersion. The model consists of two submodels, namely: for the mean and for the dispersion. Our focus is on the selection of the covariates for each submodel. Our Monte Carlo evidence reveals that the joint selection of covariates for the two submodels is not accurate in finite samples. We introduce two new model selection criteria that explicitly account for varying dispersion and propose a fast two step model selection scheme which is considerably more accurate and is computationally less costly than usual joint model selection. Monte Carlo evidence is presented and discussed. We also present the results of an empirical application. 相似文献