Auditing biochemical research data: A case study

There is little documentation in the literature of how to conduct a data audit of laboratory research. The purpose of such an audit is to verify the extent to which the published data agree with the corresponding laboratory notebook data and whether the data thus examined appear to be sufficient to justify the published conclusions. A case study is presented, which demonstrates a step by step approach towards conducting a data audit of a published research study.     

Revisions of the Fish Invasiveness Screening Kit (FISK) for its Application in Warmer Climatic Zones,with Particular Reference to Peninsular Florida

The initial version (v1) of the Fish Invasiveness Scoring Kit (FISK) was adapted from the Weed Risk Assessment of Pheloung, Williams, and Halloy to assess the potential invasiveness of nonnative freshwater fishes in the United Kingdom. Published applications of FISK v1 have been primarily in temperate‐zone countries (Belgium, Belarus, and Japan), so the specificity of this screening tool to that climatic zone was not noted until attempts were made to apply it in peninsular Florida. To remedy this shortcoming, the questions and guidance notes of FISK v1 were reviewed and revised to improve clarity and extend its applicability to broader climatic regions, resulting in changes to 36 of the 49 questions. In addition, upgrades were made to the software architecture of FISK to improve overall computational speed as well as graphical user interface flexibility and friendliness. We demonstrate the process of screening a fish species using FISK v2 in a realistic management scenario by assessing the Barcoo grunter Scortum barcoo (Terapontidae), a species whose management concerns are related to its potential use for aquaponics in Florida. The FISK v2 screening of Barcoo grunter placed the species into the lower range of medium risk (score = 5), suggesting it is a permissible species for use in Florida under current nonnative species regulations. Screening of the Barcoo grunter illustrates the usefulness of FISK v2 as a proactive tool serving to inform risk management decisions, but the low level of confidence associated with the assessment highlighted a dearth of critical information on this species.     

Understanding Normative Growth and Development in Aging

No abstract available for this article.     

Community-Based Long Term Care

Through the Living at Home Program (LAHP) 20 communities across the United States have developed cooperative networks involving multiple health and social services providers. The LAHP networks have sought to increase access to care, reduce duplication of services, and identify and fill service gaps. The LAHP national evaluation is examining the inter-organizational linkages that have been implemented by the networks and the characteristics of 1500 clients enrolled for care. Preliminary findings at the midpoint of the evaluation indicate that the networks have moved from a reliance on informal communication and coordination mechanisms at baseline to the use of more formal structural mechanisms over time. Despite the lack of formal targeting criteria, the networks have enrolled a quite frail elderly population. Future analyses will examine variability across the networks in client targeting and service utilization, In order to shed light on the need for utilization review of community based long term care.     

The classification accuracy of four problem gambling assessment instruments in population research

Improved methodology was used to re-examine the weak correspondence between problem and pathological gamblers identified in population surveys and subsequent classification of these individuals in clinical interviews. The SOGS-R, the CPGI, the NODS and the Problem and Pathological Gambling Measure (PPGM), as well as questions about gambling participation and expenditures, were administered to a total of 7272 adults. Two clinicians then assessed each person's status, based on comprehensive written profiles derived from these questionnaire responses. Instrument classification was then compared to clinical classification. All four instruments correctly classified most non-problem gamblers (i.e. had good to excellent sensitivity, specificity and negative predictive power). However, the PPGM was the only instrument with good classification of problem gamblers (i.e. excellent sensitivity and positive predictive power). The CPGI and SOGS-R had weak positive predictive power and the NODS had only adequate sensitivity and positive predictive power. Improvement in the classification accuracy of the CPGI occurred when a 5+ cut-off was used and when a 4+ cut-off was used with the SOGS. In general, the classification accuracy of the NODS, SOGS and CPGI is better than prior research suggested but overall accuracy is still modest. With adjusted cut-offs, all three instruments are reasonably congruent with clinical ratings.     

General m-esttmators and applications to bounded influence estimation for non-linear regression

In this paper, we study the M-estimators in the case that λ_F:(β)=E_F:(φ(Z,β))=0 has more than one solution, We show that the numerical iterative procedures converge and that the resulting estimators are consistent and asymptotically normal. We apply them to the non-linear regression models, and then, we find an optimal M-estimate among those that have bounded gross error sensitivity.     

On Calculations Involving the Maximum Cell Frequency

We describe a method of computing the cumulative distribution function of the maximum and minimum cell frequencies in sampling distributions commonly encountered in the analysis of categorical data.The procedure is efficient for exact or approximate calculation in both homogeneous and non-homogeneous cases, is non-recursive, and does not require Dirichlet integrals.Some related statistical problems are also discussed.     

Siting Noxious Facilities: A Test of the Facility Siting Credo

Over the past decade it has become increasingly difficult to site noxious facilities, despite the fact that there is a growing need to do so. To address this problem, a set of guidelines for a fairer, wiser, and more workable siting process—the Facility Siting Credo—was developed during a National Facility Siting Workshop in 1990. This paper presents an empirical test of these guidelines. A questionnaire based on the Credo was completed by stakeholders in 29 waste facility siting cases, both successful and unsuccessful, across the United States and Canada. Using an independent determination of outcome (success), a preliminary ranking of the importance of various Credo principles was obtained. The data reveal that establishing trust between the developer and host community is an important factor in facilitating the siting process. The siting process is most likely to be successful when the community perceives the facility design to be appropriate and to satisfy its needs. Public participation also is seen to be an important process variable, particularly if it encourages a view that the facility best meets community needs. Moreover, a siting process where communities volunteer to host facilities is an approach that holds promise for meeting many of these key success criteria.     

Global and multiple test procedures using ordered p-values—a review

This paper reviews global and multiple tests for the combination ofn hypotheses using the orderedp-values of then individual tests. In 1987, Röhmel and Streitberg presented a general method to construct global level α tests based on orderedp-values when there exists no prior knowledge regarding the joint distribution of the corresponding test statistics. In the case of independent test statistics, construction of global tests is available by means of recursive formulae presented by Bicher (1989), Kornatz (1994) and Finner and Roters (1994). Multiple test procedures can be developed by applying the closed test principle using these global tests as building blocks. Liu (1996) proposed representing closed tests by means of “critical matrices” which contain the critical values of the global tests. Within the framework of these theoretical concepts, well-known global tests and multiple test procedures are classified and the relationships between the different tests are characterised.     

Detection of interactions in experiments on large numbers of factors

One of the main advantages of factorial experiments is the information that they can offer on interactions. When there are many factors to be studied, some or all of this information is often sacrificed to keep the size of an experiment economically feasible. Two strategies for group screening are presented for a large number of factors, over two stages of experimentation, with particular emphasis on the detection of interactions. One approach estimates only main effects at the first stage (classical group screening), whereas the other new method (interaction group screening) estimates both main effects and key two-factor interactions at the first stage. Three criteria are used to guide the choice of screening technique, and also the size of the groups of factors for study in the first-stage experiment. The criteria seek to minimize the expected total number of observations in the experiment, the probability that the size of the experiment exceeds a prespecified target and the proportion of active individual factorial effects which are not detected. To implement these criteria, results are derived on the relationship between the grouped and individual factorial effects, and the probability distributions of the numbers of grouped factors whose main effects or interactions are declared active at the first stage. Examples are used to illustrate the methodology, and some issues and open questions for the practical implementation of the results are discussed.