Functional analysis of high-content high-throughput imaging data

High-content automated imaging platforms allow the multiplexing of several targets simultaneously to generate multi-parametric single-cell data sets over extended periods of time. Typically, standard simple measures such as mean value of all cells at every time point are calculated to summarize the temporal process, resulting in loss of time dynamics of the single cells. Multiple experiments are performed but observation time points are not necessarily identical, leading to difficulties when integrating summary measures from different experiments. We used functional data analysis to analyze continuous curve data, where the temporal process of a response variable for each single cell can be described using a smooth curve. This allows analyses to be performed on continuous functions, rather than on original discrete data points. Functional regression models were applied to determine common temporal characteristics of a set of single cell curves and random effects were employed in the models to explain variation between experiments. The aim of the multiplexing approach is to simultaneously analyze the effect of a large number of compounds in comparison to control to discriminate between their mode of action. Functional principal component analysis based on T-statistic curves for pairwise comparison to control was used to study time-dependent compound effects.     

你一定犯过的美妆错误

试图让嘴唇显得更"丰满"也许你曾听说过或尝试过用唇线笔在自然唇形的外围画上一圈以让嘴唇看起来更厚的做法.但是,说起来容易做起来难,正确的做法是,选用和你的唇膏相同颜色的唇线笔在嘴唇外围紧贴着画上一圈,而不是超出原本的唇形范围,接着还是用这支唇线笔给嘴唇上色,让唇膏和唇线融合在一起—这样即使你的唇膏掉了,你的嘴上也不会只剩孤零零的一圈线."蓝色眼影,我一辈子都不会放弃你"Sorry,蓝色眼影只适合某些人:舞会上的小女孩、70年代主题派对上的客人、不食人间烟火的超模.如果你不属于上述三种情况,那么请把它送人或者丢掉. 过度使用"提亮"产品在过去几年间,有提亮或高光效果的微粒被大量使用在化妆品中.一两样这样的产品会让女性看起来清新且光彩照人,但同时使用多种含有提亮效果的产品会让人看起来非常奇怪.想象一下"哑光"的皮肤配上一张闪闪发亮的脸的效果吧,一点也不性感,就好像你整个早上都靠在油缸边上一样.事实上,最多在三个部位选用提亮产品:颧骨、内眼角和下眼线(在内眼角没有使用的时候).     

The need for revaluation and assessment of Australian foster care programmes

This is a brief report on one aspect of a programme of applied research, in a large social welfare agency, designed to provide a basis for the evaluation of the policies, activities and structure of the agency.     

Marital conflict

During 1968, a centre for the treatment of autistic children was established in Perth, Western Australia, under the administration of the Mental Health Services. It was named in honour of Dr. Mildred Creak, whose authoritative contribution to the study of autism and her invaluable assistance in establishing this centre is thereby recognized.     

精品课程建设中教材编写的创新思路探索——普通高等教育《土壤学》(“十五”国家级规划)教材的编写体会与实践

作者结合北京市精品课程建设,在组织编写普通高等教育“十五”国家级规划教材《土壤学》的过程中,认为首先应明确教材编写的指导思想,即在课程教材体系上有所创新,在课程教材内容上有所更新,在教学方法和手段上有所革新。同时在贯彻适用性和特色两个原则的基础上,进行了一些编写思路的探索和创新实践,这包括摸清读者底细、确定专业目标、把握课程内涵、了解市场需求,及正确处理好理论与应用、深度与广度、难度与易度、传统与创新、利教与利学、知识传授与技能培养的关系。通过以上步骤,保证了教材的先进性、系统性和特色,并实现了网络教学。     

畸型文化土壤中生长的女性主义者--上海美女作家文本分析

女性文学是现当代文学研究中重要组成部分,本文从上海美女作家与上海海派文化关系的角度,运用女性主义者的视角,对卫慧等女性作家的文本进行分析,从而对海派文化土壤中诞生的女性主义作品作一个简要的评介和基本的判断。     

关于“林业生态工程学”教学的思考

该文分析了目前北京林业大学“林业生态工程学”教学中存在的问题,主要是课程内容较多,又受到其他因素的影响,在有限的教学时数内很难提高教学质量。同时教学实践环节比较薄弱,不能满足社会对学生工作能力的期待。对此,作者在教学内容、教学手段、课堂互动、个性化作业、综合能力培训等方面进行了一些探索,取得了较好的效果。该文提出了进一步教学改革的思路是“以课程设计为主线,围绕设计教授课程”,并沿着“理论—综述—专项规划设计—综合规划设计—评价报告”的路线进行教学。     

球墨铸铁曲轴的气体软氮化

柴油机的珠墨铸铁曲轴系重要零件,气体软氮化应用到球墨铸铁领域还是一种新工艺,而且比中频淬火效果好,提高了曲轴的抗疲劳和抗磨损的能力。本文对气体软氮化的基本原理、设备、工艺参数的选择和催渗方法进行了探讨,对软氮化后曲轴的尺寸形状误差、光洁度和机械性能的提高等进行了较详细的试验、分析和比较,结果,经气体软氮化后的曲轴不但尺寸形站误差符合图纸要求外,而且抛去轻微氧化层后光洁度还略有提高,曲轴安全系数由1.3～1.6提高到1.70～1.77,并且一般不再发生疲劳断(?),使用寿命比原来寿命增加3.32倍以上。