| 高效液相色谱法分析盐藻粉中维生素E的研究 |
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| 引用本文： | 马国文,李利军,刘芳伟,陈丽颖,兰辉.高效液相色谱法分析盐藻粉中维生素E的研究[J].内蒙古工业大学学报,2007,26(3):210-214. |
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| 作者姓名： | 马国文 李利军 刘芳伟 陈丽颖 兰辉 |
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| 作者单位： | 内蒙古工业大学化工学院 呼和浩特010051(马国文，李利军，陈丽颖，兰辉)，兰太药业 呼和浩特010000(刘芳伟) |
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| 基金项目： | 内蒙古工业大学大学生科技创新基金 |
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| 摘 要： | 本文利用高效液相色谱法对盐藻粉中维生素E含量进行测定,确立了一套完整的测定盐藻粉中维生素E的分析方法.实验采用高效液相色谱C 18柱,以甲醇为流动相,流速1 mL/min,将维生素E分离,经紫外检测器检测.维生素E标准曲线的回归方程R=0.99994,本测定方法的变异系数为1.15%,回收率93.0%~107.0%.通过对皂化时间、KOH用量、焦性没食子酸用量以及蒸发温度等因素的分析,确定了样品制备最佳实验条件.正交实验表明焦性没食子酸用量和KOH用量是影响样品制备的显著性因素.
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| 关 键 词： | 高效液相色谱 盐藻粉 维生素E 分析 |
| 文章编号： | 1001-5167（2007）03-0210-05 |
| 收稿时间： | 2006-11-29 |
ANALYSIS OF VITAMIN E IN DUNALIELLA SALINA POWDER BY HPLC |
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| MA Guo-wen,LI Li-jun,LIU Fang-wei,CHEN Li-ying,LAN Hui.ANALYSIS OF VITAMIN E IN DUNALIELLA SALINA POWDER BY HPLC[J].Journal of Inner Mongolia Polytechnic University(Social Sciences Edition),2007,26(3):210-214. |
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| Authors: | MA Guo-wen LI Li-jun LIU Fang-wei CHEN Li-ying LAN Hui |
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| Abstract: | The concentration of vitamin E in Dunaliella Salina has been analyzed by way of HPLC.A complete set of analytical methods for vitamin E is established.Chromatography conditions are:HPLC with a C18 column,and methanol used as mobile phase,with flow rate being 1 mL/min.Vitamin E is thus separated and detected by use of UV detector.The standard linear regression equation of vitamin E is R=0.99994.The variation coefficient of this method is 1.15%,and the recovery ratio is from 93.0% to 107.0%.By analyzing the saponification time,the dosage of KOH,and the dosage of pyrogallol,etc,the optimal saponification conditions are determined.The orthogonal experiment shows that the dosage of pyrogallol and KOH is the main factor that affects saponification. |
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| Keywords: | HPLC Dunaliella Salina powder vitamin E analysis |
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