| 一种赖氨酸衍生整体柱的制备与评价 |
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| 引用本文： | 李金祥,谢文辉,房晶. 一种赖氨酸衍生整体柱的制备与评价[J]. 鲁东大学学报, 2010, 26(2) |
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| 作者姓名： | 李金祥 谢文辉 房晶 |
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| 作者单位： | 辽宁师范大学,化学化工学院,辽宁,大连,116029 |
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| 摘 要： | 采用自由基引发、原位聚合的方法,制备了甲基丙烯酸缩水甘油酯-乙叉二甲基丙烯酸酯(GMA-EDMA)整体柱,用铜保护的赖氨酸与整体柱上GMA的环氧环发生反应进行衍生化,制备了一种利用配体交换作用分离氨基酸的毛细管液相色谱整体柱,并对流动相pH、离子强度和乙腈比例等因素进行了优化,成功分离了苯丙氨酸、色氨酸和酪氨酸.
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| 关 键 词： | 毛细管液相色谱 配体交换作用 赖氨酸衍生 氨基酸分离 |
Preparation and Evaluation of a Lysine-bonded Monolithic Column |
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| LI Jin-xiang,XIE Wen-hui,FANG Jing. Preparation and Evaluation of a Lysine-bonded Monolithic Column[J]. Ludong University Journal (Natural Science Edition), 2010, 26(2) |
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| Authors: | LI Jin-xiang XIE Wen-hui FANG Jing |
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| Affiliation: | LI Jin-xiang,XIE Wen-hui,FANG Jing(School of Chemistry , Chemical Engineering,Liaoning Normal University,Dalian 116029,China) |
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| Abstract: | A novel monolithic column for capillary liquid chromatography was prepared by insitu free radical polymerization of glycidyl methacrylate(GMA) and ethylene dimethacrylate(EDMA),and the prepared monolithic column was subsequently functionlized based on a ring opening reaction of epoxide groups with Cu-lysine in solution.Phenylalanine,tryptophan and tyrosine were separated through the ligand exchange interaction between amino acid and cooper after optimized several separation conditions such as pH,ionic stren... |
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| Keywords: | capillary liquid chromatography ligand exchange interaction lysine-bonded amino acid separation |
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