| 基于热可逆水凝胶包埋技术的活细胞、模型生物体、寄生动物的高容量成像 |
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| 作者姓名： | Roy EDWARD |
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| 摘 要： | 活体生物、组织切片、肿瘤球状体以及细胞,往往由于其具有很高的能动性或受布朗运动的支配,因此对高容量成像方面提出了特殊的要求,通常难以实现高分辨率的成像。科学家们试图采用黏附表面和固定化基体(如低熔点的琼脂糖和甲基纤维素)的方法来克服这些问题,但是这些技术也存在一些缺陷,通常并不适用于处理活体生物和细胞。为了解决这些问题,CyGELTM和CyGEL持续热可逆水凝胶封固剂得以开发,并为高级显微技术的发展提供思路。
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| 关 键 词： | 高容量 活细胞 生物体 包埋技术 球状体 固定化 寄生动物 成像 水凝胶 热可逆 |
High-Content Imaging of Live Cells,Model Organisms,and Parasites Using Thermoreversible Hydrogel Mountant Technology |
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| Roy EDWARD.High-Content Imaging of Live Cells,Model Organisms,and Parasites Using Thermoreversible Hydrogel Mountant Technology[J].Journal of Fujian Agriculture and Forestry University,2010(6). |
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| Authors: | Roy Edward |
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| Abstract: | Live organisms,tissue pieces,tumor spheroids,and cells present particular problems in regard to high-content imaging being motile or subject to Brownian motion. Attempts have been made to address these issues by using adherent surfaces and immobilizing matrices such as low-melting-point agarose and methyl cellulose. These have several technical limitations that make them unsuitable for general use with living organisms and cells. CyGELTM and CyGEL Sustain thermoreversible hydrogel mountants have been develo... |
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