应用改进的荧光原位杂交技术(FISH)定量分析单一细胞内miRNA的表达

在过去的十年,我们对miRNA生源和机理的理解得以快速发展;然而,许多关于miRNA表达与细胞功能如何关联的基本问题依然存在。为了能在未来实现对这个层次的理解,可能需要将单细胞水平miRNA的丰度进行定量,从而绘制细胞表型关系。本文介绍一种高灵敏度并且高效的miRNA检测方法——LNA-ELF-FISH,可以在原位的单一细胞中看见单个miRNA分子

Quantification of miRNA Expression in Individual Cells Using a Modified FISH Approach

Over the past decade,there have been rapid advances in our understanding of miRNA biogenesis and mechanism; however,many fundamental questions still remain in regard to how miRNA expression is connected to cell function. In order to achieve this level of understanding,it will likely be necessary to quantify miRNA abundance at the single-cell level,so that correlations can be drawn with cell phenotype. LNA-ELF FISH,a highly sensitive and specific method for miRNA detection,allows single miRNA molecules to be...