| 毛细管电泳法分离测定饮料中色素含量的研究 |
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| 引用本文： | 巫素琴,温亚波,邹兰,杜建中.毛细管电泳法分离测定饮料中色素含量的研究[J].湛江师范学院学报,2009,30(6):73-77. |
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| 作者姓名： | 巫素琴 温亚波 邹兰 杜建中 |
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| 作者单位： | 湛江师范学院化学科学与技术学院/湛江师范学院;广东高校新材料工程技术开发中心,广东,湛江,524048 |
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| 摘 要： | 建立了毛细管电泳法直接分离测定饮料中日落黄、胭脂红、柠檬黄的方法,研究了缓冲溶液种类、浓度、pH值、电压等对分离的影响.在波长247 nm,分离电压15 kV,pH=10.0,10 mmol.L-1NaH2PO4-10 mmol.L-1Na2B4O7缓冲溶液中,日落黄、胭脂红、柠檬黄在8 min内得到了较好的分离,用于测定市售饮料中三组分含量得到满意结果.
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| 关 键 词： | 毛细管电泳 日落黄 胭脂红 柠檬黄 |
Study on the Separation and Determination of Synthetic Pigments in Beverages Using Capillary Electrophoresis |
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| WU Su-qin,WEN Ya-bo,ZOU Lan,DU Jian-zhong.Study on the Separation and Determination of Synthetic Pigments in Beverages Using Capillary Electrophoresis[J].Journal of Zhanjiang Normal College,2009,30(6):73-77. |
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| Authors: | WU Su-qin WEN Ya-bo ZOU Lan DU Jian-zhong |
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| Institution: | (School of Chemistry Science and Technology,Zhanjiang Normal College,Zhanjiang,Guangdong 524048,China/Development Center for New Materials Engineering and Technology in Universities of Guangdong,Zhanjiang Normal College,Zhanjiang,Guangdong 524048,China) |
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| Abstract: | We have developed a capillary electrophoresis method for direct separation and measurement of the sunset yellow,ponceaue and tartrazine in beverages.The effects of the voltage,pH,solution concentrations on the separation were investigated.The results showed that three components in Na_2B_4O_7（10 mmol/L） NaH_2PO_4（10mmol/L） can be separated in 8 min at pH=10.0,U=15kV and UV=247nm.This provides a good method to detect the beverages. |
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| Keywords: | capillary electrophoresis Sunset Yellow Ponceaue Tartrazine |
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