| 紫外分光光度法测定食品中香兰素的含量 |
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| 引用本文： | 韦寿莲,赵建芬.紫外分光光度法测定食品中香兰素的含量[J].肇庆学院学报,2008,29(5):31-33. |
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| 作者姓名： | 韦寿莲 赵建芬 |
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| 作者单位： | 肇庆学院,化学化工学院,广东,肇庆,526061 |
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| 摘 要： | 采用紫外分光光度法测定食品中香兰素的含量。研究了pH值、溶剂、缓冲体系、表面活性剂等对吸收光谱和吸光度的影响。结果表明：以乙醇为溶荆，在pH值为8.0的NaHCO3-NaOH冲体系中，在不添加表面活性剂的条件下测定食品中香兰素的含量，线性方程为y=0.1167x＋0.2542，线性相关系数R=0.9992，线性范围为0.40-12.00mg／L。平均回收率大于97.0％，RSD小于2.0％，说明该方法结果可靠，重现性好。
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| 关 键 词： | 香兰素 紫外分光光度法 食品 |
Determination of Vanillin in Food by Ultraviolet Spectrometry |
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| WEI Shoulian,ZHAO Jianfen.Determination of Vanillin in Food by Ultraviolet Spectrometry[J].Journal of Zhaoqing University (Bimonthly),2008,29(5):31-33. |
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| Authors: | WEI Shoulian ZHAO Jianfen |
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| Institution: | (Faculty of Chemistry and Chemical Engineering, Zhaoqing University, Zhaoqing Guangdong 526061, China) |
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| Abstract: | A simple method for the detection of vanillin in food by UV speetrophotometry was devel- oped. Effects of pH, organic solvents, buffer type and concentration, surfactant type and concentration have been investigated. The optimum conditions of detection for vanillin was 0.5 mol/L NaHCO3-Na2CO3（pH 8.0） buffer with ethanol as a solvent. The wavelength for detection was set at 343.65 nm. Linear equation was y=0.1167x＋0.2542（R=0.9992）,linear range was covered from 0.40 mg/L to 12.00 mg/L. The average recovery of method was more than 97.0%, while RSD was less than 2%. The results were accurate and reliable. |
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| Keywords: | Vanillin Ultraviolet Spectrophotometry food |
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