| 芋艿茎尖诱导成苗成芋的组培技术研究 |
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| 引用本文： | 杨秀坚,孙诚志.芋艿茎尖诱导成苗成芋的组培技术研究[J].湛江师范学院学报,2003,24(6):66-70. |
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| 作者姓名： | 杨秀坚 孙诚志 |
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| 作者单位： | 1. 湛江师范学院,生命科学与化学学院,广东,湛江,524048
2. 湛江市农业技术推广中心,广东,湛江,524043 |
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| 摘 要： | 以芋艿茎尖为试材，研究培养基和不同光照条件对芋艿茎尖诱导成苗和试管苗的诱导成芋的影响。结果表明：茎尖诱导成苗的较理想培养基为：NS 3％蔗糖 6-BA5．0mg／L-1 NAA0．02mg／L-1(单位下同)；分化培养基为：NS 3％蔗糖 6-BA3．0 NAA1．0；诱导成芋培养基为：NS 8％蔗糖 6-BA1．0 NAAO．5；自然光培养比日光灯培养的试管苗明显偏高，粗壮，试管芋的块茎明显偏大，但分化苗的丛芽数较少。
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| 关 键 词： | 芋艿 茎尖 自然光照 诱导 试管苗 试管芋 组织培养 培养基 光照条件 |
| 文章编号： | 1006-4702(2003)06-0066-05 |
| 修稿时间： | 2003年10月8日 |
The Tissue Culture Technique of Inducing Seedling and Taro from Taro's Stem- tip |
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| YANG Xiu-jian,SUN Cheng-zhi.The Tissue Culture Technique of Inducing Seedling and Taro from Taro''s Stem- tip[J].Journal of Zhanjiang Normal College,2003,24(6):66-70. |
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| Authors: | YANG Xiu-jian SUN Cheng-zhi |
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| Institution: | YANG Xiu-jian~1,SUN Cheng-zhi~2 |
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| Abstract: | This paper investigates the influence of the different media and different illuminations on inducing seedlings and test-tube seedlings from taro's stem-tips.The experimental results show that the ideal medium of inducing seedlings,differentiating seedlings and inducing taros fromtaro's stem-tips is NS+3%sucrose+6-BA5.0+NAA0.02,NS+3%sucrose+6-BA3.0+NAA1.0 and NS+8%sucrose+6-BA(1.0)+NAA0.5(drug unit:mg/L-1) respectively.The test-tube seedlings in the natural illumination culture are obviously higher and robuster than those in the fluorescent lamp culture,and the test-tube taro's bulbs in the natural illumination culture are also bigger than those in the influorescent lamp culture while the number of clump-buds of differentiating seedlings is smaller in the above mentioned same comparison as mentioned above. |
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| Keywords: | taro natural illumination inducing test-tube seedling test-tube taro |
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