分光光度法测定茶制品中儿茶素含量的研究

依据儿茶素与香草醛的显色反应原理,用分光光度法对茶制品中儿茶素含量进行测定,建立香草醛-盐酸法测定茶制品中儿茶素含量的方法.结果表明显色剂和催化剂的添加量及反应时间等因素对测定均有影响.该法检测波长为500nm,反应时间为15h,反应温度(20±1)℃,以儿茶素为标准样品,线性范围10-lOOug/mL,线性关系良好(r=O.999),相对标准偏差<1％.该法操作简便,其它辅助材料无干扰,可用于原料药和制剂中儿茶素含量的测定.

Studies on Content Measurement of Tea Catechins of Tea Produces by Spectrophotometry

A spectrophotometry is used to determine the content of tea catechins of tea produces according to the principle of display reaction between tea catechins and vanillin. In addition, a method of vanillin - hydrochloric acid is established to determine the content of tea catechins from the produces. The results show that the additive quantity of display reagent, catalyst and reactive time, etc. are able to affect the determining data. Determining wavelength of 500nm, reaction time of 15h, and reaction temperature of 20 ?1 are adopted in determining content of tea cate-chins. Moreover, the linear ranging from 10 to 100 (g/mL), the favorable linear relation having r = 0.999 and the relative standard deviation that is lower than that of 1 % , are observed while the tea catechins are employed as a stan-dard . This analyzing method has the features of easy operation and no interference with other assisted materials, which is suitable to measure the content of tea catechins from the material drug and the preparation.