六种神经递质在纽虫脑神经节的免疫组织化学定位

运用免疫组织化学方法研究了促肾上腺皮质激素(ACTH)、生长抑素(SOM)、P物质(SP)、γ-氨基丁酸(GABA)、乙酰胆碱酯酶(AChE)、五羟色胺(5-HT)在短无头沟纽虫(Baseodiscus curtus)脑神经节内的分布情况.实验结果表明:ACTH免疫阳性反应细胞零散分布于脑神经节胞体区,胞体大小约12μm×6μm;脑神经节少数中型细胞呈SOM免疫阳性反应,胞体大小约15μm×9μm;脑神经节胞体区周边部可见许多大型细胞呈SP免疫阳性反应,胞体大小约19μm×15μm,胞体区内侧可见少量小型细胞呈SP免疫阳性反应,胞体大小约11μm×6μm;GABA免疫阳性反应细胞主要集中在脑神经节腹侧区,胞体大小约13μm×8μm;5-HT免疫阳性反应细胞集中在脑神经节胞体区的腹外侧部,胞体大小约13μm×8μm;脑神经节胞体区周边大部分细胞呈强的AChE免疫阳性反应.

Immunohistochemical Localization of Six Neurotransmitters in Cerebral Ganglion of Nemertean

The localization of adrenocorticotrophic hormone(ACTH),somatostatin(SOM),substance P(SP),γ-aminobutyric acid(GABA),acetylcholinesterase(AChE) and serotonin(5-HT) in cerebral ganglion(CG) of a nemertean(Baseodiscus curtus) was studied with immunohistochemical method.The result showed that the ACTH positive reaction cell about 12 μm×6 μm was located scatteredly in soma area of CG,a few medium-size cells about 15 μm×9 μm present SOM positive reaction,many large cells about 19 μm×15 μm in periphery and a few small cells about 11 μm×6 μm in medial part of CG presented SP positive reaction,the GABA positive cell about 13 μm×8 μm was located mainly in ventral part of CG,the 5-HT positive cell about 13 μm×8 μm was located mainly in ventrolateral part of CG and most cell located near peripheral of CG presented AChE positive reaction.