| 蒙古冰草与航道冰草杂交F1代染色体加倍植株过氧化物同工酶分析 |
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| 引用本文： | 郝峰,刘翠玲,郝水源.蒙古冰草与航道冰草杂交F1代染色体加倍植株过氧化物同工酶分析[J].河套大学学报,2010(4). |
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| 作者姓名： | 郝峰 刘翠玲 郝水源 |
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| 作者单位： | 中国农业科学院研究生院;内蒙古河套排水事业管理局;河套大学; |
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| 摘 要： | 采用聚丙烯酰胺垂直板电泳技术对蒙古冰草与航道冰草正、反交杂种F1代与其染色体加倍植株的POD同工酶酶谱表征进行了分析。结果表明:在分蘖期、抽穗期2个不同发育阶段,蒙古冰草×航道冰草正交杂种F1代染色体加倍植株、航道冰草×蒙古冰草反交杂种F1代染色体加倍植株与蒙古冰草×航道冰草正交杂种F1代植株、航道冰草×蒙古冰草反交杂种F1代植株、蒙古冰草亲本、航道冰草亲本的POD同工酶酶带在数目、位点及强弱上均存在一定差异,同工酶酶谱表征可作为正、反交杂种F1代与其染色体加倍植株在蛋白质水平相互识别的重要依据;在对不同植物材料鉴定时从几个不同的生育阶段做同工酶酶谱对比分析,要比单一生育阶段更能反映各材料间酶谱的遗传差异性,提高其鉴定结果的可靠性。
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| 关 键 词： | 蒙古冰草 航道冰草 正反交杂种 染色体加倍植株 同工酶酶谱表征 |
An Analysis of Reciprocal Hybrids F1 Between Agropyron Mongolicun and A. Cristatum cv. Fairway and Its Chromosome Doubling Plant Isozyme |
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| Authors: | Hao Feng Liu Cuiling Hao Shuiyuan |
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| Institution: | Hao Feng1,Liu Cuiling2,Hao Shuiyuan3 (1.Postmaster Institute of China Agricultural Science Academy 2. Hetao Drainage Management Bureau,Linhe 015000,3. Hetao University,Linhe 015000) |
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| Abstract: | The proxidase (POD) and esterase (EST) isozyme characteristics of reciprocal hybrids F1 between Agropyron mongolicum and A. Cristatum cv. Fairway and its chromosome doubling plant were analyzed by using vertical slab polyacyrylamide gel electrophoresis technology. The results showed that there were certain differences in number, locus and intensity of the proxidase (POD) and esterase (EST) isozyme bands among the reciprocal hybrids F1 between A. mongolicum and A. chritatum cv. Fairway and its chromosome dou... |
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| Keywords: | agropyron mongolicum a chritatum cv Fairway reciprocal hybrid chromosome doubling plant isozyme xymograms phenolypes |
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