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豇豆品种同工酶聚丙烯胺凝胶电泳凝胶浓度的筛选
引用本文:雷刚,胡志辉,陈禅友.豇豆品种同工酶聚丙烯胺凝胶电泳凝胶浓度的筛选[J].江汉大学学报(社会科学版),2001,18(6):28-31.
作者姓名:雷刚  胡志辉  陈禅友
作者单位:江汉大学,农学系,武汉,430065
摘    要:用凝胶浓度分别是7.5%和6.0%的聚丙烯酰胺凝胶系统对12个豇豆品种的酯酶(EST),淀粉酶(a-AMY)和过氧化氢酶(CAT)同工酶作电泳分析,将两种不同浓度凝胶系统的电泳图谱作对比,得出豇豆品种的淀粉酶同工酶聚丙烯酰胺凝胶电泳应选择低浓度凝胶系统(6.0%),酯酶同工酶,过氧化氢醋同工酶聚丙烯酰胺凝胶电泳应选择高浓度凝胶系统(7.5%),才能得到较高质量的凝胶电泳图谱的结论。

关 键 词:豇豆  同工酶  凝胶浓度  聚丙烯酰胺凝胶电泳  酯酶  淀粉酶  过氧化氢酶
文章编号:1006-639X(2001)06-0028-04

Ridding on gel chroma of Polyacrylamide gel elecrophoresis in the Isozymes of Asaparagus bean Cultivars
LEI Gang,HUZhi-hui,CHEN Chan-you.Ridding on gel chroma of Polyacrylamide gel elecrophoresis in the Isozymes of Asaparagus bean Cultivars[J].Journal of JIanghan University:Social Sciences,2001,18(6):28-31.
Authors:LEI Gang  HUZhi-hui  CHEN Chan-you
Abstract:The esterase (EST), amylase (a-AMY) and catalase (CAT) of 12 main cultivars of Asparagus bean were analyzed by polyacrylamide gel electrophoresis. Two gel chroma were balanced. The results from their balance analysis showed that the amylase ought to choose lowchroma gel system (6.0%), the esterase and catalase ought to choose high chroma gel system (7.5%).
Keywords:Asparagus bean  Isozymes  Gel chroma
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