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用于牛乳中抗生素检测的嗜热乳链球菌的快速分离及签定
引用本文:卜秀娟,张燕,王作昭,王二雷,王宏艳,刘凯. 用于牛乳中抗生素检测的嗜热乳链球菌的快速分离及签定[J]. 吉林工程技术师范学院学报, 2012, 28(8): 67-69
作者姓名:卜秀娟  张燕  王作昭  王二雷  王宏艳  刘凯
作者单位:吉林大学军需科技学院,吉林长春,130062
摘    要:本文研究了从发酵酸乳中快速分离纯化嗜热乳链球菌的方法.文中采用涂布分离法在溴甲酚紫指示剂的牛乳营养琼脂平板上可以快速的分离嗜热链球菌.经过镜检和发酵试验鉴定所分离出的菌株为纯种嗜热乳链球菌,可用于牛乳中抗生素检测.

关 键 词:溴甲酚紫牛乳营养琼脂  嗜热乳链球菌  发酵  革兰氏染色

The Rapid Isolation and Identification of Thermophilic Streptococcus Lactic for Detection of Antibiotics in Raw Milk
BU Xiu-juan , ZHANG Yan , WANG Zuo-zhao , WANG Er-lei , WANG Hong-yan , LIU Kai. The Rapid Isolation and Identification of Thermophilic Streptococcus Lactic for Detection of Antibiotics in Raw Milk[J]. Journal of Jilin Teachers Institute of Engineering and Technology(Natural Sciences Edition), 2012, 28(8): 67-69
Authors:BU Xiu-juan    ZHANG Yan    WANG Zuo-zhao    WANG Er-lei    WANG Hong-yan    LIU Kai
Affiliation:(College of Military Supplies Science and Technology,Jilin University,Changchun Jilin 130062,China)
Abstract:This paper studies the method of the rapid isolation and purification of thermophilic streptococcus lactic from fermented milk.Coating partition method is used to separate thermophilic streptococcus lactic on the bromcresol purple indicator of cow’s milk nutrient agar plate and the effect is quite well.The result showed that this method can attain its well separation.The bacterial strain isolated is identified as pure thermophilic streptococcus lactic through microscopic examination and fermentation test,so this method can be used for the detection of antibiotics in milk.
Keywords:bromocresol purple milk nutrient agar  thermophilic streptococcus lactic  fermentation  Gram-staining
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