三七三醇皂苷和胞二磷胆碱联用对局灶脑缺血再灌注大鼠的脑保护作用

目的研究三七三醇皂苷(PTS)、胞二磷胆碱(CDP-c)联用对大鼠局灶脑缺血/再灌注的神经保护作用。方法用大脑中动脉栓塞法制作短暂性(90min)局部脑缺血/再灌注模型,观察PTS、CDP-c单用及联用7d后,大鼠脑梗死体积、caspase-3表达及凋亡细胞数。结果和对照组相比,PTS组、CDP-c组及两药联用组脑梗死体积较小,caspase-3表达、凋亡细胞数均较少,并有统计学意义。两药联用组和PTS组、CDP-c组相比,脑梗死体积较小、caspase-3表达、凋亡细胞数较少(P<0.05)。PTS组和CDP-c组相比各指标无统计学意义。结论PTS和CDP-c单用对短暂脑缺血/再灌注模型可能具有神经保护作用。PTS与CDP-c联用对短暂性局造脑缺血/再灌注的神经保护可能有协同作用。

Cerebral Protective effects of combine PTS and CDP-c on transient focal cerebralischemia-reperfusion in rats

Objective To investigate the neuroprotective effects of PTS and CDP-c in rat model of transient focal cerebral ischemia-reperfusion. Methods The model of acute reperfusion injury after transient cerebral ischemia in rat was made by middle cerebral artery occlusion. Expression of caspase-3 and apoptotic cells in infarction were detected, and infarct volumes were assessed after PTS, CDP-c and their combined administration for 7 days. Results Compared to placebo group, infarct volume of PTS,CDP-c and combination groups were significantly reduced,and the number of caspase-3 positive cells and apoptotic cells were significantly fewer as well,respectively(P<0.05).meanwhile compared to both PTS group and CDP-c group infarct volume of combination group were significantly reduced,and the number of caspase-3-positive cells and apoptotic cells were significantly fewer, respectively. However, there was no significant difference between PTS group and CDP-c group. (P>0.05). Conclusion PTS and CDP-c may be respectively exert a neuroprotective effect during the transient experimental focal cerebral ischemia-reperfusion model.The results of this article demonstrates a possible synergistic neuroprotection effect of PTS and CDP-c after transient experimental focal cerebral ischemia-reperfusion.