钙敏感受体促进小鼠长骨的生长及软骨矿化

目的:探讨钙敏感受体(calcium sensing receptor,CaSR)对小鼠长骨及软骨矿化的直接作用?方法:利用骨移植的方法将出生后3 d同窝的野生型和CaSR基因敲除小鼠的股骨胫骨移植至同父母来源的8周龄的野生型小鼠背阔肌中?生长4周后,取出股骨和胫骨进行X线和组织学分析?结果:CaSR基因敲除小鼠骨骼生长严重阻滞,并且其生长板的软骨肥大细胞带显著宽于野生型小鼠的软骨肥大细胞带(P < 0.001)?结论:CaSR具有直接促进长骨生长的作用,并且能够促进肥大软骨细胞矿化和骨化?

Calcium sensing receptor improves long bone formation and cartilage mineralization of mice

Objective:To determine the direct action of calcium sensing receptor (CaSR) on both long bone and cartilage mineralization of mice. Methods:The femur and tibia of both 3-day-old wild-type and CaSR gene knockout mice from the same brood were transplanted into the latissimus dorsi muscle of 8-week-old wild type mice with the same parents of the 3-day-old mice. After 4 weeks,the femur and tibia were analyzed with X-ray and histology methods. Results:CaSR knock-out mice exhibited growth retardation and the hypertrophic zone of cartilage was obviously wider than that of the wild-type mice (P < 0.001). Conclusion:CaSR have a direct promoting action on long bone growth,and can stimulate the mineralization and ossification of hypertrophic chondrocytes.