TWIST基因和蛋白与宫颈癌发生发展关系的研究

目的:检测TWIST基因在宫颈癌组织和宫颈正常组织中的表达,探讨其与宫颈癌发生?发展的相关性及在宫颈癌诊疗中的意义?方法:收集56例宫颈癌组织和35例宫颈正常组织,用实时定量PCR检测TWIST mRNA的表达;用Western blot检测TWIST蛋白的表达,并分析TWIST mRNA在宫颈癌中的表达水平与临床病理因素及高危型HPV病毒载量相关性?结果:实时定量PCR结果显示宫颈癌组织中TWIST mRNA表达高于宫颈正常组织,差异具有统计学意义(P < 0.01);Western blot在蛋白质水平上证实了这一点(P < 0.01)?宫颈癌组织中TWIST的表达与淋巴结转移相关(P = 0.022),但与年龄?FIGO分期?肿瘤大小?病理类型?肿瘤分化程度无关(P均> 0.05),Logistic回归分析显示,高水平的TWIST mRNA表达是淋巴结转移的独立危险因子(P < 0.05)?而TWIST基因的相对表达水平与高危型HPV病毒载量无相关性(r = 0.205,P > 0.05)?结论:宫颈癌组织中TWIST的表达上调,且与是否有淋巴结转移相关,TWIST的表达可能对宫颈癌的发生?发展及转移有重要作用?

Significance of TWIST gene and protein in the occurrence and development of cervical cancer

Objective:To detect the expression of TWIST gene in cervical cancer and normal cervical tissues,explore the correlation between TWIST gene and the development of cervical cancer,and assess the clinical application of TWIST gene detection for cervical cancer diagnosis. Methods:The primary tumor tissues from 56 cervical cancer patients and 35 normal cervical tissues were collected. The expression of TWIST mRNA was detected by Real-time PCR. TWIST protein levels were determined by Western blot. The correlation analysis was conducted to assess the relationship between TWIST expression and clinical pathological parameters,load of high risk human papilloma virus (HPV). Results:The results of Real-time PCR showed that the expression rate of TWIST gene in the cervical cancer tissues was significantly higher than that in normal cervical tissues (P< 0.01),which was verified by the expression of TWIST protein levels detected by Western blot(P < 0.01). TWIST expression in the cervical cancer tissues was correlated with lymph node metastasis(P = 0.02) ,but not with age,FIGO stage,tumor size,pathological type and the differentiation grade of tumor (both P > 0.05). Logistic regression analysis showed that high level of TWIST mRNA expression was an independent risk factor for lymph node metastasis (P < 0.05). There was no correlation between TWIST expression and load of high risk HPV in the cervical cancer group(r = 0.205,P > 0.05). Conclusion:TWIST expression in the cervical cancer tissues was up-regulated,and associated with lymph node metastasis. It may play an important role in the pathogenesis and metastasis of cervical cancer.