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内蒙古绒山羊KAP6-2全长cDNA的特征分析
引用本文:马勇.内蒙古绒山羊KAP6-2全长cDNA的特征分析[J].阴山学刊,2007,21(3).
作者姓名:马勇
作者单位:包头师范学院生物科学与技术学院 内蒙古包头014030
摘    要:选择10月份周岁绒山羊体侧部皮肤构建cDNA质粒文库,随机挑选克隆提取质粒,用M13正向通通用引物,测插入片段的序列,得到636个序列。与Genebank数据库比对后,发现有1个序列与小鼠的KAP6-2基因的期望值小于le-10,对其预期编码蛋白的氨基酸进行分析,建议绒山羊KAP6-2具有HGTps(高苷/酪氨酸蛋白,high glycine/tyrosine proteins)与富含半胱氨酸蛋白之间的中间特征。

关 键 词:绒山羊  全长cDNA

Characteristic Analysis of KAP6-2 Full Length cDNA of Inner Mongolia Cashmere Goat
MA Yong.Characteristic Analysis of KAP6-2 Full Length cDNA of Inner Mongolia Cashmere Goat[J].Yin Shan Academic Journal,2007,21(3).
Authors:MA Yong
Abstract:Choosing the side skin of one years old cashmere goat in October to construct cDNA plasmid library,selecting some clones randomly to pick-up the plasmid,measuring the inserted sequences with M13( ) primer,then getting 636 sequences. Compared the sequences with Gene bank,finding a sequence is similar to KAP6-2 of mouse,the expectation is less than le-10. With analysis of amino acid sequences of the proteins,I suggest that KAP6-2 of cashmere goat has intermediate characteristics between HGTps and cysteine-rich proteins.
Keywords:KAP6-2
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