| 氨基酸分析的新方法——积分脉冲安培检测-高效阴离子交换色谱法 |
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| 引用本文： | 于泓,牟世芬.氨基酸分析的新方法——积分脉冲安培检测-高效阴离子交换色谱法[J].福建农林大学学报(哲学社会科学版),2003(1). |
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| 作者姓名： | 于泓 牟世芬 |
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| 作者单位： | 中国科学院生态环境研究中心，Dionex中国有限公司应用研究中心 北京 100085 哈尔滨师范大学化学系 哈尔滨 150080，北京 100085 |
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| 摘 要： | 介绍一种新型氨基酸分析方法——采用积分脉冲安培检测的高效阴离子交换色谱法(HPIC-IPAD) 对氨基酸的阴离子交换色谱分离和积分脉;中安培检测进行了较详细的讨论 对方法的应用做了简要介绍。该方法与其它方法比较,无须进行柱后或柱前衍生反应,对氨基酸可以直接进行分析,灵敏度较高 大多数氨基酸的检测限小于1pmol,线性范围可以达到3个数量级以上。
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| 关 键 词： | 氨基酸 阴离子交换色谱法 积分脉冲安培检测 |
A New Method for Analysis of Amino Acids -Anion-Exchange Chromatography with Integrated Pulsed Amperometric Detection |
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| Yu Hong,Mu Shifen
Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences.A New Method for Analysis of Amino Acids -Anion-Exchange Chromatography with Integrated Pulsed Amperometric Detection[J].Journal of Fujian Agriculture and Forestry University,2003(1). |
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| Authors: | Yu Hong Mu Shifen
Research Center for Eco-Environmental Sciences Chinese Academy of Sciences |
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| Institution: | Yu Hong,Mu Shifen
Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences. Beijing. 100085,China
Application and Research Center of Dionex China Co. Ltd.,Beijing,100085,China
Department of Chemistry,Harbin Normal University,Harbin,150080,China |
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| Abstract: | A new method for analyzing amino acids by anion-exchange chromatography with integrated pulsed amperometric detection is introduced and discussed in detail. The application of the method is briefly described. Compared with traditional methods, this method can analyze amino acids directly without post-column or pre-column derivatization. The new method is highly sensitive with the detection hmit of <1 pmol. The range of linearity is over three orders of magnitude for most common amino acids. |
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| Keywords: | Amino acids Anion-exchange chromatography Integrated pulsed amperometry |
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