| 单个分子光学检测仪器的搭建和检验 |
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| 引用本文： | 关英华,洪莲,Urs P Wild,赵新生. 单个分子光学检测仪器的搭建和检验[J]. 福建农林大学学报(哲学社会科学版), 2003, 0(2) |
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| 作者姓名： | 关英华 洪莲 Urs P Wild 赵新生 |
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| 作者单位： | 北京大学分子动态与稳态结构国家重点实验室及化学学院化学生物学系，北京大学分子动态与稳态结构国家重点实验室及化学学院化学生物学系，ETII Zrich，Switzerland (II-8022)，北京大学分子动态与稳态结构国家重点实验室及化学学院化学生物学系 北京 100871，北京 100871，北京 100871 |
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| 基金项目： | 国家重点基础研究发展规划(TG1999075305)，国家自然科学基金(29953001,20128003)资助项目 |
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| 摘 要： | 在Nikon TE300倒置显微镜的基础上 搭建并检验了两套单个分子检测系统 即远场共焦显微镜和远场宽场照明显微镜。实验证明,所搭建的仪器达到了单个分子检测的水平 为利用单个分子光学检测技术研究生命科学中的问题奠定了基础。
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| 关 键 词： | 单个分子检测 共焦荧光显微镜 宽场荧光显微镜 |
Construction of Optical Microscope for Single Molecule Detection |
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| Guan Yinghua Hong Lian Urs P. Wild Zhao XinshengState Key Laboratory of Molecular Dvnanuc and Stable Structures. Construction of Optical Microscope for Single Molecule Detection[J]. Journal of Fujian Agriculture and Forestry University, 2003, 0(2) |
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| Authors: | Guan Yinghua Hong Lian Urs P. Wild Zhao XinshengState Key Laboratory of Molecular Dvnanuc and Stable Structures |
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| Affiliation: | Guan Yinghua Hong Lian Urs P. Wild Zhao XinshengState Key Laboratory of Molecular Dvnanuc and Stable Structures. Department of Chemical Btology. College of Chemtstrv,Peking University. Beijing,100871,China ETH Zurich. Swuzerland CH-8022 |
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| Abstract: | Based on Nikon TE300 Microscope we have constructed an optical microscope which can delect single molecule in the mode of either confocal fluorescence or wide-field fluorescence microscope. The microscope has been characterized with some typical behaviors in single molecule detection. The successful construction of the apparatus for single molecule detection makes it possible to study biological systems at single molecule level. |
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| Keywords: | Single Molecule Detection Confocal Fluorescence Microscope. Wide-field Fluorescence Microscope |
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