| 蓝猪耳叶片高频率再生体系的建立 |
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| 引用本文： | 王瑛华,陈刚,陈雄伟. 蓝猪耳叶片高频率再生体系的建立[J]. 肇庆学院学报, 2007, 28(2): 58-61 |
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| 作者姓名： | 王瑛华 陈刚 陈雄伟 |
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| 作者单位： | 肇庆学院生物学系 广东肇庆526061 |
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| 基金项目： | 肇庆学院校科研和教改项目 |
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| 摘 要： | 以蓝猪耳(Torenia fournieri Linden)叶片为外植体,研究多种因素对蓝猪耳不定芽诱导及不定根形成的影响,并进行了条件优化,建立了蓝猪耳叶片高频率离体再生体系.结果表明:芽诱导与基本培养基成分关系不大,而与植物生长物质的浓度和种类有关,其中以MS+6-BA 2 mg/L+NAA 0.2 mg/L状态最佳;不定根形成受到植物生长物质和培养基中离子浓度的共同影响,最佳生根条件为1/2 MS+IBA 0.2 mg/L.
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| 关 键 词： | 蓝猪耳 植株再生 |
| 文章编号： | 1009-8445(2007)02-0058-04 |
| 修稿时间： | 2006-09-15 |
Establishment of a High Frequency Efficient Regeneration System from Leaf in Torenia fournieri Linden |
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| WANG Yinghua,CHEN Gang,CHEN Xiongwei. Establishment of a High Frequency Efficient Regeneration System from Leaf in Torenia fournieri Linden[J]. Journal of Zhaoqing University (Bimonthly), 2007, 28(2): 58-61 |
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| Authors: | WANG Yinghua CHEN Gang CHEN Xiongwei |
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| Abstract: | In order to establish a high frequency regeneration system from leaf in Torenia fournieri Linden,some conditions about shooting and rooting had been compared.The highest shoots differentiation was obtained on the MS medium supplemented with 6-BA 2 mg/L and NAA 0.2 mg/L.The results showed that regeneration of adventitious roots was controlled by hormones and salt concentration,and 1/2MS+IBA 0.2 mg/L was the optimum rooting medium. |
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| Keywords: | 6-BA NAA IBA |
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